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Semi-Quantitative Analysis of Optical Transparency 3µm Porous Membranes
After seeing in previous posts how optically transparent the SiO2 membranes are, I went about seeing if I could quantify this in comparison to the SiN membranes and also CoStar PET track-etched membranes. In order to do so, I ‘seeded’ red 1µm beads (in 1X PBS) onto each type of membrane (the SiO2 and SiN…
Disposable PDMS diaphragm pumps
In order to include pumps on the prototype blood analyzer cartridges, it was necessary to implement a small pump using the existing knife-cut PDMS layer layup technique. The diaphragm architecture was selected due to ease of operation and minimal additional part requirements. CAD model of pump in Autodesk Inventor Bottom view of fabricated pump. The…
Diffusion Cell New Designs
ByJimHere are two new designs for the new diffusion cells. Design_2 could be a better solution as it separates the two connections and uses screws to make the wire contacts to outside circuit. Please feed back any ideas to improve these designs. Dave and I probably will start working on it some time this week….
Modeling Oxygen and Urea transfer in Nanomembrane Devices
Introduction Extracorporeal membrane oxygenation (ECMO) devices are used to supply oxygen to patients when they are unable to get sufficient oxygen through their lungs. Miniaturizing an ECMO device could be beneficial for neonatal patients, as it would require less blood to prime the device and operate efficiently at a lower overall flow volume. Dialysis is…
Human Albumin/Cytochrome C diffusion cell
I ran another diffusion cell last week using Human Albumin and Cytochrome C. I increased the volume of PBS because the last time I ran the gel for this mixture, the band for cytochrome c was very prominent. Out of the three chambers of the diffusion cell that I ran, only one of them actually…
Permeability w/ PBS
In a first attempt to reduce the electrostatic drag that might be slowing water transport, I ran an experiment with 1x PBS. The membranes were first ozone treated and then assembled into the SepCon inserts. From these first trials, it appears that PBS does pass through more quickly than DI water.
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This belongs in a notebook. Data on the blog does not need to be polished, but it should in a form that communicates a result to others. If everyone uploaded their rawest data, the blog would be unbearable to read.