In continuation of the previous post, its been further observed that O2 RTP treated samples remain unchanged whereas the Ar RTP treated samples started to discolor after a week, as shown in the following images:
In order (left to right)
1. Ozone+Ar RTP
2. Ozone+O2 RTP
3. Control (Untreated)
4. Ar RTP
5. O2 RTP
This experiment compares the metal developer used in Integrated Nano Technologies’ device to to the flow properties of water. Using the pressure cell at 3 psi, with no allyl treatment, for 10 minutes, and 200 microliters of liquid, I pushed water and developer through our membranes and examined the difference in flow rates. All of…
Introduction Sepsis related projects had a decent presence at this years Biomedical Engineering Society (2019). I will detail a few of the talks I found most relevant to our group and research. Nanomedicine for Acute Criticial Illness by Dr. Jacob Brenner (U Penn Medical) Dr. Brenner is a practicing physician working on ICAM-1, PECAM-1, and…
Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test. The results of the third growth experiment is shown below. After 86 hours the cells…
Kelly & I finally succeeded in getting a measurement of the SU-8 scaffold change in size upon liftoff. Kelly, lifted off a sample (~0.5 x 2 cm) using the XeF2 dry etch and transferred the sample to a glass slide with pdms bonded to it. The slide was then placed on top of the printed…
Two more adhesion tests were run recently, one on Tuesday and one on Wednesday. For both tests cells were imaged after five hours. Fibroblast trial 3 was the first test where the optimal cell density (assuming 100% of cells are detached by trypsin and 100% attach to the substrate) was measured using a hemacytometer. Of…
These curves were all generated using the following package of matlab functions and scripts: (Sieving copy – run Find_Sieving_Coefficient_boltzmann.m – it’s set up to generate concentration profiles right now). The program begins with a ‘standard separation’ that uses the following characteristics: Pore diameter: 58 nm Particle diameter: increases from 20 nm to 56 nm in 2 nm increments…
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Excellent – an expected difference!! So how long do they need to be stable for? We are going to be balancing a variety of factors now, so we need to determine what our targeted stability time is. Were the unstripped membranes stable for over a week? If so, how long?
Were the O2 RTP membranes a little wrinkled after the treatment? How long was the treatment – probably a few minutes? I have a feeling that they probably have a lower burst pressure if they have wrinkled up. Still, we can dial back the heat or O2 flow so they don’t wrinkle, but this will probably reduce their ultimate stability. We may also want to try the nitrogen treatment, although I’m not sure cells will still stick to them at that point. We should also try annealing before stripping the mask oxide.
Lots to discuss!!