Capillary Tubing and Adapter
Just wanted to let everyone know that the fused silica capillary tubing and adapter needed to connect them to the pump tubing have been ordered and should be in hopefully by Friday (Monday at the latest).
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This experiment is to determine if the time between the SC1 clean and when the wafers get deposited matter. Currently there is suspicion that an SC1 from an RCA clean should at RIT should be done right before deposition, . So what is done typically is that lithography steps are done weekly at RIT to…
Pressurized Gold Separations
Motiviation and Background: Nakul and I have been performing a lot of DNA filtration and attempted DNA shearing experiments. We found that large DNA fragments (>1000 and >2000 bp) pass through ~30-40 nm max pore size membranes when pressurized to 10 psi (even in low salts). This is in contrast to the Bernhard data where…
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fornasiero2008
Make Sure to Filter Salts Before Passing Them Through Sepcons
Previously, I had gotten some inexplicable results showing that increasing salt concentration decreases the flow through a membrane. This makes no sense (and goes against our previous data) because we would expect electrostatic forces to counteract the flow, an effect called counterelectroosmosis by Zydney: “The situation in the presence of a net fluid flow through…
Evidence of functional sodium/hydrogen exchangers in single EVs captured on NPN
ByJimThanks to our collaborators at the University of Chicago we have now used NPN to demonstrate sodium/hydrogen exchange functions at the level of single EVs. This is first-of-a-kind evidence of protein function in individual EVs. It will be officially published in Communcations Biology and will soon be located at this DOI. What follows are key…
October Journal Club: Single Nanopore Particle Analysis
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