Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is only the prototype, there must be upside space for improvement.
Introduction My previous post focused on attempts to reproducibly generate collagen IV with human umbilical vein endothelial cells (HUVECs) so that we would be able to establish protocols for basement membrane protein detection and degradation measurement. Following my presentation on November 18 2020, there were some questions regarding whether or not the observed collagen IV…
It was speculated that the rate of diffusion through a charged membrane had a direct relationship with pressure – as pressure increases, the kinetic energy of the charged particles is more likely to overcome the electrostatic repulsion of the membrane, until at a high enough pressure the repulsive force is small enough to be ignored….
There has been some experimentation recently on trying to form very large pores in thick membranes. We have found that a high temperature anneal (1000 C) along with a fast ramp rate (100 C/s) w/o the susceptor can create these large voids. It seems that the distribution widens as we push the cut-off up. The…
What has been accomplished thus far: Able to detect BSA on NC membrane with the use of Ponceau S stain With PDMS array on NC, no BSA leaks through the area covered by PDMS Initial trial done with 10:1 ratio of elastomer base: curing agent. Later 3:1 and 5:1 ratios done. 3:1 is too sticky…
The following is a plot of rhodamine diffusion for the experimental system at various time point: In this figure, the rhodamine approaches equilibrium, as defined as a filtrate/retentate value of 1. The 9x well chips have a larger active area and quicker diffusion. I have performed simulations of this system using COMSOL, and in this…
I measured the I-V characteristics of our coated membranes on Wed. at RIT. JP observed the measurement. The semicon parameter analyzer in the test lab is “old” but isexactly what we need! Roughly speaking, our coated membraneshave resistance about 2k Ohm, depending on the thicknesses ofmetal films (this is based on pure Ti coated samples,…