Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is only the prototype, there must be upside space for improvement.
I ran a 30 hour urea clearance with 100% serum through the single slot dialysis device. The now standard protocol was run. The single slot chip was annealed in the RTP for robustness then clamped into the acrylic/PDMS flow fixture. Ethanol was pumped through the chip for 1 hour, then degassed PBS was pumped for…
Hey folks, Science Saturday is this Saturday and Sunday at the Rochester Science Center. We need volunteers to man our tables from 12-4pm on both Saturday and Sunday. Please indicate in the comments of this post if you can volunteer, and when you can volunteer. The tentative schedule is as follows: 12-1 Saturday – Greg,…
Continuing the studies on neutrophil transmigration in devices with embedded parylene membranes of different pore sizes and thicknesses, three pore sizes (1.0 um, 1.4 um and 1.7 um) and two thicknesses (0.7 um and 1.2 um) were used for these sets of experiments. Thickness ratio of 1.2 um to 0.7 um roughly correlated with…
Separation of Nanoparticles using surface acoustic radiation force: Surface acoustic waves (SAW) can be defined as mechanical waves which propagate on the surface of an elastic medium which most of its energy is concentrated in the surface. It’s been used in many different biological applications such as separation of cells, tumor cells, microvesicles, nano and…
Introduction After completing my experiments on burst pressure of HD membranes, I started the experiments on finding the burst pressure of 3-channel nitrite chips by using the airgas cylinder. This experiment is to investigate if soaking in viscous fluid will significantly weaken the membrane and lead to burst. The chip was placed on the center…
Based on the results obtained yesterday with the closed flow cell we reran another trial today. The closed flow system we tested today was bound using UV Ozone treatment, surprisingly this system created a relatively strong bond despite our resent results. Once the UV Ozone treatment was complete and the system had bound vacuum grease…