Time lapse movie of fibroblast spreading on the nanomembrane was captured over a period of ~ 8 hours.
I was having some problem uploading the video here, thus have done so in the NRG knowledge page under the cell culture manuscript.
Using streaming potential data collected via the flow device Jess created, I have attempted to recreate the Zydney findings for membrane zeta potential. Ag/AgCl electrodes were used in 10mM KCl, mimicking identically the method outlined in Zydney. Data are below: The streaming potential for both trials of PES membranes varied significantly after 60 minutes in…
I realized last week that I had made a pretty substantial mistake with my quantification which involved my mass standards. Half way through running the gels for the previous study I had accidentally started making my mass standards at a higher concentration but counting them in the quantification as their initial value. The bottom line…
In my previous post, there still appears to be a discrepency between the calculated pore statistcis and the experimental permeabilities. I explored what I could do with the pore processing to bring the experimental closer to the calculated permeabilities. I set the minimum pore cutoff higher (15nm) and tried to remove any pores that did…
I ran new experiments over the weekend in which I tested NaCl at 10, 25, 60, and 155mM. I also ran filtrations in the presence of 10mM LiCl and 10mM CsCl. These experiments were performed with chips from wafer # 024. I am only providing the Filtrate / Retentate ratios. It is obvious that the…
Michael and I have been working to create cell channels of varying widths and heights to test out a concept used in a paper we recently read as a group. This work claims to see cell cords in 12 hours, so no media renewal is necessary. The channels were made by layering and bonding…
This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells. These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days. Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but one survived this 7-day cell culture experiment. These cells were plated on the bottom on…
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You definitely have the right idea here. Its close, but there are a few issues.
1) The cells do not look healthy in the last few frames. Did the media dry out or something. 2) The imaging isn’t high enough quality. We need to move the old camera back and use fluorescence or work on the Zeiss at 37C. Or perhaps the light level was just too low.
I agree Jim.
The media was drying out and had to be replenished. Also we have been having issues while looking at the cells closely using the canon camera. Next time would do that using the old camera.