I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs.
Fibroblast spreading on glass 1
Fibroblast spreading on glass 2
Time lapse movies for fibroblasts spreading on pncSi are on their way.
I derived the governing equation for the volume transfer from SepCon devices in the wet/wet (EQ) format. It is … where the equilibrium volume for the cup can be calculated apriori using the cross sectional areas of the cup and the surrounding annulus … with the total volume of interest given by and the time…
I first reported the crystallization of free-standing amorphous silicon membranes in this post in January. The last experiment was done inside the susceptor (top and bottom piece). This week, I annealed a set of amorphous 15 nm and 30 nm with the susceptor top off. This allowed for the direct heating of the films. One…
Since precision printing FITC KODE molecules into the windows of our 3 slot chips I have begun testing both the uniformity and the functionality of surface coatings of these molecules. In pursuit of coating uniformity I have tested several different methods of surface coating to determine which method provides the greatest uniformity. In this same…
Introduction Sepsis refers to an uncontrolled systemic inflammatory response which can result from bacterial blood infections. We have long been interested in developing a sepsis model using the μSiM-BBB platform because the neuroinflammation stemming from sepsis can cause severe BBB disruption. For the majority of our stimulation experiments we have relied on cytomix, a cocktail…
I guess there was a mistake in the last permeability plot. The real data for the 4 bar plot should look like this: Unfortunately that doesn’t look so good anymore. The third bar though is the one that I originally processed to have many more pores (w705). The reprocessing gets it down in the 8%…
Yesterday afternoon I made a new batch of 10:1 (base : curing agent) PDMS and put it in the oven at 80C for two hours to allow it to cure faster (compared to the three days it takes at room temperature). Once the PDMS was cured Henry used the UV Ozone system to again try…
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These look very nice. I hope we can get pnc-Si movies that look similar.