Dave Fang posted a poster in 2010 about particles found in DI water. I encountered the same issue during the nano-pocket permeability experiment. He said, “I’ve become increasingly suspicious of membrane clogging during the … permeability experiments.” I conducted an experiment with ultrapure water (UPW), typically filling a 1L glass container from a UPW machine….
Introduction: In the previous post, we demonstrated our capability to fabricate the nano-pocket membrane and effectively capture and release particles of various sizes. In this post, our focus shifts to exploring the membrane capability. We will focus on the membrane modeling (parking point, the plateau region)of the nano-pocket membrane. To have an overview of the…
Introduction: Building upon previous research conducted by the Gaborski and McGrath labs, this poster introduces a novel approach to isolate extracellular vesicles (EVs) based on size. The earlier work involved the development of a tangential flow membrane capture system to address isolation challenges. In this extension, a nano-geometry membrane is fabricated to selectively filter out…
The fabrication of nano pocket membrane to capture and release the EV faced challenges during the etching step. There was a suggestion after the last NRG post to use RIE-2000 located in the UR. This post shows the update on the methodology of creating the nano pocket membranes and the preliminary results after using the…
Starting in July of 2021, we began a new idea fabricating the nano pocket membrane to capture and release the EVs. From then until now we have successfully fabricated nano pocket membranes by using a lithography method. We faced challenges in parylene etching and resolve it by using two etching machines, i.e. Trion Phantom…
Goal: Is to produce a membrane has pockets to capture the extracellular vesicles EV’s. Membrane structure: Figure 1 shows the membrane structure. The membrane will have two pores sides: upper and lower. The pores on top are bigger than the bottom and the diameter of the pores should be similar to or larger than the…