W648: Burst Pressure and the Return of Contamination?

This is a side view schematic of the experiments I’m setting up in Mike and Derek’s stir cell. The membrane separates two wells. In the well where the membrane is flush with the wafer I add 300uL 5nm gold in H2O. On the other side I add my buffer and a stir bar. Adding a…

This is a retrial of the previously outline negative adsorption assay.  I was a little more careful with cuvettes this time around (there are very significant differences in the plastic cuvettes) and I spread out the same amount of solution over a larger area.  If exposed to more surface, more protein should adsorb and leave…

Let’s get a blast-from-the-past and go through a bunch of drawers in the lab!  I spoke with Jim and it seems like it might be best to schedule it during next regularly slotted Tuesday NRG mtg.  So let’s plan on meeting downstairs in the lab on 2-19 at 9:45 am. Also, I bought an assortment…

w565 is permeable to rhodamine. I tested two chips using 100uM rhodamine using a time step of .5 minutes. Click on pictures to see animations. position (1,-1) – While trying to do this first sample, MATLAB crashed and I was forced to take the movie about 7 minutes after the start. There is also a…

Membrane chips were amino-silanized with APTES in the YES system.  I performed the standard electroosmosis tests with these chips.  Here are the flow rates in comparison with non treated and oxidized membranes: Here are the calculated zeta potentials: As you can see, amino silane reduces the rate of electroosmosis and the zeta potential. We would expect…

Josh and I are taking a closer look at how the samples burst and what different factors can affect the strength of the membrane. We are specifically looking at minimum dimension, area, and perimeter. A wafer was designed that contained different sized samples. One of the three factors were held constant while the other two…