Let’s get a blast-from-the-past and go through a bunch of drawers in the lab! I spoke with Jim and it seems like it might be best to schedule it during next regularly slotted Tuesday NRG mtg. So let’s plan on meeting downstairs in the lab on 2-19 at 9:45 am.
Also, I bought an assortment of o-rings from Harbor Freight (I love that store!). I put them in a drawer under Henry’s bench space where I’ve found other o-rings.
The goal of this project is to determine the viability of PEG as a structural support for large area nano porous membranes. This would enable lift-off vs. the current method of etching through the wafer. The ideal properties are that the structure can withstand an aqueous solution for many days without peeling off or dissolving,…
Hello Everyone, So Shannon and i have been working on studying the effect of exosomes in different conditions for wound healing assays and random migrations. This post is about one of our experiments (wound healing) to give you an update on our data and what we’re able to get with Henry’s matlab code. How the…
last week I ran some tests using a single 100K microcon filter; this week I preformed essentially the same tests but instead of testing one filter over the entire pressure range I used a new filter at each pressure. I tested the permeability at 1.35psi, 21.63psi, 118.7psi, and 267.1psi; each pressure was tested using three…
This is just an update for the experiments Tejas and I have been working on for the past two weeks: First, we tried incorporating 20 μl of Geltrex (10 mg/ml, diluted with DMEM from stock concentration: i.e. 15.6 mg/ml) in the back of a non-porous chip with 0.02 μm blue fluorescent nano-spheres (1:10 dilution from stock concentration)….
The 300K nanosep membranes that Jess ordered came in so I ran a single set of three membranes at 21.63psi to compare their hydraulic permeability values to those of the 100K microcon membranes. As expected the 300K nanosep membranes have a much larger hydraulic permeability then that of the 100K microcons; approximately a factor of…
My last post was the first experiment I performed with rat lung epithelial cells on transwells. It seemed like there was vacuolization but it was delayed compared to endothelial cells (and only happened on 1 of 2 pnc-Si transwells). Here, I did a repeat with a lower seeding density (25000 cells/cm2) and with SC613. The…
Comments are closed.
I forgot I was in a proposal defense Tues morning. Should be done by 10:30. Lets meet in the lab at 11:00.