Tecan and Transport
Before starting my transport project with the use of the Tecan, it was important to see how the Tecan worked with the sepcon and transwell kept in the well plate. The goals from this experiment were to:
1. Test for fluorescence from the plastic.
2. See if fluorescence from the apical side has an affect on the reading from the basolateral end.
The set-up and materials are as follows:
*Tecan
*receiver: HBSS with Hepes
*donor: Fluoroscein
*5 wells
-Sepcon
-Sepcon without membrane
-Transwell
-HBSS
-Blank
Here is a screen shot of the set-up for the Tecan. Readings are done in a circular motion to avoid the dip in the sepcon.
Data:
The experiment should be done a few more times due to the unexpected steep rises/falls. However, this experimental set-up will probably work for transport experiments: I shouldn’t have to remove the sepcon everytime I need a reading.