Journal club

In this post I show the sieving coefficients of simulations with a thin membrane, a thick membrane, or a no membrane case as they approach equilibrium.  The simulations were carried out for 48 hours, and plotted for 1 hour time points.  Both thin and thick membrane had the same pore distribution – monodisperse 10 nm…

Silver chloride electrodes are useful for microfluidic devices since they can generate current below one volt. This allows them to function without fear of electrolysis confounding experimental results by way of gaseous clogging. Several different methods for coating silver mesh were tested to determine which would be ideal for future experimentation. These methods were as…

Hi all, For quite some times we have been trying to design a diffusion-based chemotaxis chamber that operates under an environment of 37 degrees Celsius in temperature. One main obstacle that we face is the evaporation of the 2 uL of rhodamine 6 G (R6G) that we load on the well openning of the membrane…

XTT is a colorimetric assay for measuring cell viability.  Supposedly, it gets taken up by cells and is reduced by mitochondrial activity intracellularly into water-soluble orange formazan crystals.  The readout is the absorbance of these orange crystals. I used XTT to get standard curves of HUVECs in 24-well plates first.  Here’s the data from 2…