While my focus will be on packaging a single multi-channel chip device, I have captured here an initial concept for packaging multiple channels. The technique required is the application of verticle PDMS structures to direct blood and dialysate to and from the stacked levels of chips.
Introduction Tissue barriers regulate the absorption of nutrients, maintain homeostasis, and prevent toxins from entering. As previously introduced, trans-epithelial/endothelial electrical resistance (TEER) measurements offer a non-destructive and real-time method to quantitatively evaluate the integrity of tissue barriers. During the process, a small AC current (10 µA, 12.5 Hz) is applied across the barrier while the…
Update 8/15/14: Replaced the plots with updated versions including more data for NPN (now n = 3) and cellulose (n = 4,) as well as new high-flux polyethersulfone membrane data (n = 3.) I also dropped one clearly erroneous datapoint from the NPN dataset to dramatically cut down the standard deviation. — This is a followup…
Dave deposited a series of wafers last week at different temperatures. SC 166 -SC 173 were deposited between RT and 300C. These wafers had Jess’ E.O mask pattern ( circular sepcon) with single , 3,6 and 9 squares. The wafers were mostly pinhole free.In order to determine if deposition temperature affected the nature/ strength of…
Previously I ran a chemical stability study of samples from W613, which has a 30 nm thick pnc-Si layer. In that study complete failure of untreated samples was observed by 18 hours in cell culture, which was not a significant improvement from sample lifetime of 15nm thick pnc-Si. To see if RTP treatment helped the…
Last week I ran cell culture growth experiments on RTPed (800C 5 minutes in Ar) samples from W567 (single slit 100um x 1000um). For this study I tracked discoloration w/cells, membrane stability w/cells, and the bead assay w/cells to see if we can predict if a membrane used in cell culture is intact by monitoring…
We manufactured a wafer specifically for SERS experiments in the hope of recapturing larger pore distributions/pore densities that we observed in some of the prototype runs of NPN. Natively, it’s a little thicker, and a little less porous than the 1085 material. By partially etching the membranes from the backside, we should be able to…