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Zetapotential of untreated pnc-Si varies with pH
EDIT – 2/10/14 – Adding 1 mM phosphate buffer fixes the problems outlined in this post. I’ve been having some trouble taking consistent pH measurements with the new pH probe. Prior to Thanksgiving I made a spectrum of 10 mM KCl buffers (at 4, 6, 7, 8, and 10 pH units) for use in my…
Rotation Project Complete: Dialysis of cytochrome c across a commercial membrane
Dan Steiner Rotation Summary Abstract The purpose of this rotation project was to develop and test a clampable system for eventual use in scalable nanomembrane dialysis systems. The nanomembrane dialysis system will be miniaturized into a compact, wearable hemodialysis device that will provide more effective human dialysis treatment (Johnson et al. 2013). For initial tests, a…
Back to NPN nanofilter experiments
Just a quick update on my status: I got the NPN pre-filters from Greg in Montreal and finished testing 15 of them last week, so this week I will start doing additional experiments. I plan to explore the size separation capabilities in KCl, which should give even tighter distributions than LiCl. I don’t plan on…
PSi and pnc-Si degradation, again
I repeated the first degradation experiment (posted here) to confirm the results. I also tracked discoloration of the samples this time. As before, I used 1X PBS, but this time, the entire assay was performed in the 37C oven. Discoloration pictures: The PSi sample starts with a new rainbow coloration and then completely discolors to…
Diffusion boosts sieving coefficients in dead-end filtration through pnc-Si
ByJimOne of the features of the gold nanoparticle separations in SepCons that was under-appreciated by the reviewers at Science was the fact that we could get very high sieving coefficients in the neighborhood of the cut-off. For 5nm gold the sieving coefficient was 0.8 (i.e. the filtrate was 80% as concentrated as the stock solution)…
HUVEC in Sepcons
I’ve been doing some preliminary studies with HUVEC in commercial transwells and on pnc-Si in the Sepcon/transwell hybrid format. The thinness of pnc-Si will enable better microscopic examination of cells than other membrane systems (if pnc-Si is flat). I thought it would be interesting to compare commercial transwells to the pnc-Si “hybrid transwell” format in 24-well plates via…