MEMs and membranes: a Liver Assist Device

I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was too dark, as seen by the image below. Harold Smith gave me some Ponceau S…

As I mentioned in my last post, I will be performing protein diffusion tests through CytoVu assemblies (from one well to the other) and measuring the amount of protein that is still in solution when the system reaches equilibrium in order to determine the amount of protein that is adsorbed to the assembly and thus…

I guess there was a mistake in the last permeability plot.  The real data for the 4 bar plot should look like this: Unfortunately that doesn’t look so good anymore.  The third bar though is the one that I originally processed to have many more pores (w705).  The reprocessing gets it down in the 8%…

This is probably not of general interest, but for the sake of full disclosure, I have spent considerable time working with our wafer supplier (Virginia Seiconductor) to resolve some issues regarding the surface quality of their wafers. It would have been much more productive to simply switch suppliers, but unfortunately, they are the only place…

Hi everyone! I’m sifting through the mass of data I collected during my last month and am compiling posts to update everyone. This post shows the latest with the microfluidic chamber that Henry and I designed.  In my first post regarding this device, I showed microparticle and blood flow over cultured endothelial cells (bEnd3).  In…

Key Ideas I took a wafer with ~400 chips of varying square window sizes, ran my MgF2 process, and recorded the pressures it took to burst them. Overall yield was about 20%, most of them smaller windows. I was expecting ~50% based on my previous work. Variation in the etch step destroyed the yield. MgF2 nanomembranes…