MEMs and membranes: a Liver Assist Device
Maria Lynch will review this article by Jeffrey Borenstein and colleagues …
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Hand-cast Protein Gels: New Technique and Fresh Chemicals
In April of last year, I spent a considerable amount of time trying to visualize protein separations using SDS-PAGE (Sodium Dodecyl Sulfate-PolyAcrylamide Gel Electrophoresis) gels, and ultimately was able to get some data: I was trying to show that 15 nm pnc-Si could be used to separate BSA from cytochrome c, and while at first…
Pressurized Gold Separations
Motiviation and Background: Nakul and I have been performing a lot of DNA filtration and attempted DNA shearing experiments. We found that large DNA fragments (>1000 and >2000 bp) pass through ~30-40 nm max pore size membranes when pressurized to 10 psi (even in low salts). This is in contrast to the Bernhard data where…
Membranes with movable cutoff
Last week, we created a series of wafers that were RTPed at four different temperatures between 900 C and 1000 C. Below is a comparison of the 900 C (left) vs. 1000 C RTP (right) wafer. We see that a difference of 100 C shifts the cutoff ~10 nm. The pores are also the roundest…
W613: Retest of Stability in Cell Culture Media
I recently repeated a chemical stability test of RTPed (5 min in Ar at 800C) samples from W613, which has the 30 nm pnc-Si layer. In this study I used DMEM +10% FBS as the culture media without cells. Previously RTP treatment of samples from this wafer survived about 7 days. In this iteration of…
Closed System Device
One aspect of the blood brain barrier device is that it be a closed flow system. This would not only better mimic an in vivo environment, but also maintain Sema4D activity, negatively affecting endothelial cell junctions and providing a target for the antibody. The microporous membranes were not yet available, so a closed flow system…
micro-porous lift-off TEOS
For this experiment, 3um diameter pores were created using SimPore’s mask. Reactive Ion Etching was used to form the pores in the SiN. Both 25nm and 150nm TEOS oxides were used. An SU8 Structure of 10um lines with 100um square windows was chosen. A sample was placed in 10:1 BOE w/ surfactant for various amounts…