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More on non-uniformity
There’s been some discussion on whether furnace-annealed-only wafers are more uniform than RTP’ed ones. Today, I looked at several samples from wafer 304 (FA 1000 C) at different positions along the y-axis. (0, 5) – 25kx (0,2) – 25kx (0,1) – 50kx (0, -1) – 25kx (0, -4) – 25kx There’s a clear change in…
HUVEC and Fibroblast Growth Summary
Thus far three HUVEC and two L13T3 growth tests have been conducted on substrates including glass,pnc-Si, tissue culture plastic, and untreated plastic. Only one test with untreated plastic has been run for each cell type. Cells on Si samples are not on the slits but rather on substrate surrounding. It seems that HUVEC proliferate more…
Electron Beam Evaporation, RIT vs UR
The CHA e-beam evaporator in RIT had been used traditionally for the deposition of metal on the pnc-Si membranes. The most important factor for metal deposition on the membranes is that the nanoholes must not be blocked. So the metal deposited needs to be smooth and continuous. Ag or Au does not adhere onto Si…
Modeling EOF and Electric Field Gradient at a Microporous Membrane
I used COMSOL for finite element analysis (FEA) modeling of EOF and DEP at a microporous membrane in a PDMS microfluidic channel. Effects of two parameters were determined: Membrane thickness, and spacing between pores. The modeled membranes had a 25×25 um surface, and a thickness of either 100 nm or 500 nm. Pore diameter was…
Microfluidic control over millimeter scale alignment of collagen fibers
Type I collagen is a fibrous protein and the largest constituent of the ECM by mass. Collagen fibers are aligned across several millimeters to centimeters within load-bearing tissues, like the musculoskeletal tissue, cardiac tissue, and the cornea. Millimeter scale regions of fiber alignment are also observed in the tumor stroma and fibrotictissue. At the cell scale,…
Some results for DNA translocations through NPN pre-filters
From my last blog post, was talked about designing an assay whereby the NPN filter would exclude DNA-conjugated gold nanoparticles while allowing short DNA to pass. Since then, I (with the help of my colleague Dan Tassier) have been wrestling with gold nanoparticles to find conditions in which the nanoparticles were stable, while also allowing…