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Gel from Diffusion Chamber
Jess showed me how to use the diffusion chamber to separate proteins on the membranes. I ran a gel to see if any proteins appeared in the filtrate. The gel is posted below: The lanes are (from left): protein standard, control, retentate 1, filtrate 1, retentate 2, filtrate 2. It appears that protein diffused through…
Notes from PSST 2014
The International Conference for Porous Semiconductors Science and Technology, 2014, was last week in Benidorm, Spain. Jim was kind enough send me to represent NRG. Dave Fang gave a great talk at PSST based on pnc-Si data in 2010. Here is a pdf of all the abstracts: PSST 2014-Abstracts A quick summary would break the…
Pore Density vs. Pore Radius Graphs, 15nm width and 1000RTP
The purpose of this post is to see if there is a pattern in the Pore Density vs. Pore Radius graphs at certain manufacturing conditions. The following wafers all have widths of 15nm and RTPs of 1000. The porosities range from 6.48% to 8.33%. **** note: the y-axis label should actually read “pore density (#/nm2)”…
Gold Ladder
I obtained gold in 5nm, 20nm, 40nm, and 60nm through Mort/Oberdorster Lab. Separations were performed with fully crystallized samples from SC 073. Here are sizing and absorbance data: In Water I have tried 24 hour separations in diH2O (SC 073) with these particles. Here are the results: No 525nm peak in filtrate for 5nm sample. …
Discoloration – oven vs. incubator
A couple weeks ago, I posted some pictures that showed a non-linear “dose-response” curve for NaHCO3 and discoloration in the incubator (post). I repeated this experiment at 37C in the benchtop oven. These pictures show that there was a NaHCO3 concentration dependence on the discoloration rate in the oven. In the 2nd set of…
BBB co-culture (2)
In my last post (the first co-culture), I showed that bEnd3 cells exhibited a different morphology when they were co-cultured. I also decided that I would reverse the orientation of the co-culture in order to better visualize the endothelial barrier. That is, grow bEnd3 on the basolateral side and NG10815 on the apical side of…