Journal Club for June 26

Here are the results of diffusion based protein separations at different salt concentrations and 50kD Microcons: We see a sharp cutoff between 31 and 45kD.  Separations look like they have progressed further in 10mM salts rather than 100 mM (this is the opposite of what we see in pnc-Si).  The 100kD data may also support…

As mentioned in my previous few posts Justin and I have been working to characterize contact angles resultant from various exposures in the UV-Ozone system. Earlier this week we got our new oxygen tank installed and it appears that after using the 15min cleaning protocol (15min@150C with vacuum) noticeable decreases in contact angles can be…

Back a long time ago, I had performed some separations with the aim of showing the usefulness of pnc-Si in hemodialysis. I attempted separations of albumin (an important protein to be kept in the blood) and cytochrome c (used in hemodialysis research to cheaply approximate the toxic protein b2microglobulin) using three different membranes. Here is…

Below is an optical image that shows the amount of area loss caused by the preferential EDP etch. Based on these numbers, our most recent membrane slits measure 115 um x 2014 um.

Wafer 612 was made to mimic wafer 504: 850 C RTP, cool down, 1000 C RTP.  Wafer 615 was annealed in two steps: first to 400 C and then to 1000 C (without inbetween cool down).  Both wafers showed a lower pinhole density.  Wafer 615 had a few “traditional” round pinholes.  Wafer 612 had some…

I’ve been learning the discoloration protocol from Karl and Anant and finally have some pictures of my own to show. We got a couple of new media (RPMI and DMEM/F12) last week in order to grow the cells I’m getting from the Elder lab (rat lung cells). I did a discoloration test to see if…