Analysis of pumping burst pressures of HD membranes
Sabrina’s post showed that on a bench top, with ‘blood’ source and return at ATM, the transmembrane burst pressure was between 0.5 psi and 1.5 psi, (initial and after DI or PBS flow for four hours). Extrapolating from Burst pressure chip tests for the 10 mm long HD chips, we can expect the burst pressure to be somewhere between 1 psi and 2 psi. (Extrapolation from 1 mm – 3 mm up to 10 mm is problematic). So we are in the expected range. Also, previous PBS exposure tests show some degradation of membrane strength after exposure to PBS (single slot chips, 22 hours, 3.5 psi to 2.5 psi, n=4, sigma > 1 psi).
I still want to run more complex (protein rich fluid) but as we now know the bench-top working transmembrane pressure, I want to return to the ‘static’ burst pressure cell with an array of soaked chips to find the time-frame and effect of blood-like fluids on the membranes.
I am presently calculating and theorizing the pressures in the animal model based on the vascular pressures measured.