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Journal Club: Nanothin Coculture Membranes with Tunable Pore Architecture and Thermoresponsive Functionality for Transfer-Printable Stem Cell-Derived Cardiac Sheets
ByHenryAn application of nanothin and highly porous (NTHP) membranes for mesenchymal stem cell (MSC) differentiation in a co-culture setting. The highlight of this article is that the NTHP membrane allows direct contact of the MSC to cardial myoblast (H9C2) while still maintaining the separation of the different cell types. This ultimately leads to higher degree…
Electrospinning aligned polymeric fibers on parylene stencils
The aim of this work was to electrospin aligned polymeric fibers on parylene stencils as means to characterize the critical necessary total area and necessary opening sizes to elicit endothelial cell response in the form of cell alignment among other characteristics typically found in human cells that are adjacent to highly aligned ECM. For this…
Discoloration Study with Bacterial Media
I performed a test study to see the effect of microbial media’s : LB and BHI Broth on the membrane to check for discoloration. LB Broth : Lysogeny broth,Luria broth or Luria-Bertani broth. Obtained from Sigma and made by dissolving 20g in 1 l of D.H2O and autoclaving at 121C for 15 mins. LB is…
BSA-DDM Complex Intensity
ByMike HI took a look back through the Melvern data I collected, particularly focusing on changes in the intensity data in the BSA-DDM solution (the previously posted plots were of number data). When I compared the peak intensities for BSA, DDM, and DDM-BSA complex I noticed that the intensity peaks fall over the same size distribution…
Exploring Formlabs SLA 3D printing for cell culture applications
The initial purpose of exploring formlabs SLA 3D printer for a collaboration with Dr. Nicola Marchi (link to previous post). The purpose of the this work was gaining the ability to use a simple membrane on 3D printed platform for BBB modeling and WBC migration though endothelial (and glial cell layers) and collecting and…