Monocyte Isolation via CD14+ Selection (Protocol)

ByDan Ahmad March 17, 2021March 17, 2021

Please see the attached .PDF file for a full monocyte isolation protocol. This is for isolating human monocytes from whole blood.

Monocyte-Isolation-Protocol

memBrains | NRG

hiPSC Differentiation to Endothelial Cell Culture Method, Brain Microvascular Endothelial Cells (EECM-BMEC-like cells), Round 1
ByMolly McCloskey March 17, 2021March 19, 2021
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and develop strategies that protect against cognitive decline. To fill this gap, we are developing a…
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memBrains

Using Dual-Scale Membranes as a Model for a ‘Leaky’ BBB
ByMichelle Trempel April 4, 2023
Introduction Many neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease, are associated with an early breakdown of the blood brain barrier (BBB). This includes an increase in permeability and pericyte degradation [1]. We hypothesize that using dual-scale (nanoporous and microporous) membranes in the µSiM-BBB model will create an inherently ‘leaky’ BBB which can be used…
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memBrains | NRG | μSiM

Updates on Modeling the Vascular Basement Membrane in the μSiM-hNVU
ByLouis Widom April 25, 2022
Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of small molecules and cells. Inflammatory diseases may disrupt the BBB and promote the development of neurological disorders [1]. One potential mechanism of BBB disruption is the degradation of the extracellular matrix proteins that make up the endothelial…
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memBrains | NRG | μSiM

Daily In-Situ Permeability using mouse CMECs
ByFeyone La March 29, 2022March 29, 2022
Introduction In the Glading Lab we study Cerebral Cavernous Malformations (CCMs). In CCMs, brain capillaries develop enlarged lumens and a disrupted blood-brain barrier. The presence of a mutation in KRIT1 causes loss of KRIT1 protein function in the endothelial cells. KRIT 1 plays a role in the integrity of endothelial junctions, therefore the lack of…
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NRG | Protocols

New Alt. Membrane Release Recipe (Nitride RIE)
ByGreg Madejski May 8, 2015
I have lost many chips during my MgF2 or Au or Pt process due to yield problems with my RIE. I have wondered if the problem was due to power spikes. I have observed that my most poorly yielding etch runs usually have uneven power or power spikes during the run. Increasing window size (active area)…
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memBrains

Introduction of beads into the flow module
ByMehran Mansouri February 24, 2021March 5, 2021
1. Introduction Fluid flow is one of the key elements of vascular models for delivering nutrients and growth factors to cells as well as introducing other components like neutrophils. In post 1, we described our modular design for incorporating a flow module into the uSiM device. Then, we presented fluid flow simulation and its experimental…
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