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Goal In preparation for experimentation with hCMEC/D3, an immortalized BBB cells line, I characterized barrier formation by taking TEER. I want to find peak barrier formation to determine what day to run assays. Methods hCMEC/D3 were maintained and passaged in EGM-2 (EBM-2 + multiple gfs + gentamicin + 2.5% FBS) with growth factor concentrations matching…
ALine Capture Device In this post I will go over some of my recent work that is a continuation of Kilean’s research concerning extracellular vesicle (EV) capture and characterization. In the past, this was done using a tangential flow filtration (Figure 1) scheme and clamped devices featuring nanoporous membranes (Figure 2). These devices are straightforward…
UPDATE: A look at long and short crystal dimensions (9/1/10): DLS vs. Crystal Sizes – Newest version 8/30/10 Here is a comparison of sieving curves using DLS or estimated crystal dimensions of proteins. DLS: 24 hours sieving curve. 48 hours sieving curve. Estimated longest crystal dimension: 24 hours sieving curve. 48 hours sieving curve.
I have been developing a microsystem for Paul Dunman’s group that will allow them to flow in bacteria, immobilize them on antibodies, and then subject them to varying concentrations of antibiotics. The full system will incorporate a fluidic mixer and a NPN membrane as a way to introduce the antibiotic I have been working with…
I have just completed testing and documentation of a MATLAB GUI designed to allow anyone to make predictions of the clearance behavior of solutes though our membranes in tangential flow. I’m uploading the file here, which includes all the MATLAB code for the GUI, dependancies, and documentation. It will also appear under the resources tab on the…
I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells. Here’s a post of images. I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve been investigating the optimal arrangement of the different cell types (bend3 on top or bottom?). …