UV Ozone treated W404 sample testing (benchtop) : In EGM In L15 In DMEM In each row, the first two samples (from left) are UV Ozone treated whereas the next two are not.It can be observed that there is no significant difference in discoloration due to the treatment after ~15 hrs. Note: The starting color…
W407 (non treated) in different NaCl salt concentrations (benchtop) : At t=0 In order (from l-r)1. 0.5 M NaCl2. 1.0 M NaCl3. 2.0 M NaCl At t=24 hrs. At t=53 hrs. At t=6 hrs and t=12 hrs the chips were not found to be discolored.This was carried out to test the stability of the membranes…
Membrane stabilityNon treatedW404 samples in DMEM 15um beads on the membrane:15um beads below the slit:In order: (left to right)1. t=02. t=33. t=64. t=105. t=13 hrs.Non treated W336 samples in DMEM15um beads on the membrane:15um beads below the slit:In order: (left to right)1. t=02. t=123. t=264. t=315. t=48 hrs.Chip discolorationNon treated W404 samples in DMEMIn order:…
Above video shows the disruption of the membrane from W404 after around 12 hours in the DMEM media (benchtop). The membrane was not treated with UV-Ozone and degraded pretty early as compared to that from W336 which broke at ~28 hrs. The membranes were loaded with 15um beads to check if they were intact and…
15um beads on discolored membrane surface, having some slits where the beads are not found The localization of microbeads suggest that they have fallen across the slits in the membrane Transparent membrane with microbeads on its surface These membranes are not treated with cell media, hence are not discolored. There’s a slit which appears to…
Surface functionalization results