W407 (non treated) in different NaCl salt concentrations (benchtop) :
At t=0
In order (from l-r)
1. 0.5 M NaCl
2. 1.0 M NaCl
3. 2.0 M NaCl
At t=24 hrs.
At t=53 hrs.
At t=6 hrs and t=12 hrs the chips were not found to be discolored.
This was carried out to test the stability of the membranes in different salt conc, in order to carry out some desalting experiments.
Wafer 1148 was produced roughly around the same time that the wafer I used to make my oldest MgF2 material (wafer 1085) was produced. We know that some of the NPN material from that time was not etched all the way through, creating pores that look like a ‘bowl-with-hole’. I believe the shape of the…
Summary After success with the development and execution of the μSiM-CA (Canalicular Array; see Masters et al., Nanomedicine: Nanotechnology, Biology, and Medicine 2019), Jim wanted to develop a platform to answer some more biophysics focused questions. In order to facilitate these studies, the platform would ideally be configured to allow for live imaging of staph…
I used 100kD Millipore Celllulose membranes to separate the 2 C-dot particles. The results are similar to those I’ve obtained with pnc-Si; the 10nm particles pass while the 30nm ones do not. Cy5 10nm particles: Sulforhodamine 30nm particles: Here are the sieving ratios: Particle Ratio 10nm (abs) .60 10nm (fluor) .59 30nm (abs) .02 30nm…
I finished the first experiment to look at rhodamine transport across commercial transwell membranes. I was mainly interested if there would be enough transport into the basolateral chamber to measure the rhodamine with the spec. Experimental details: both chambers in PBS and at room temperature, spinning on the rotomix, 0.5mM initial rhodamine in donor; sample…
One of the features of the gold nanoparticle separations in SepCons that was under-appreciated by the reviewers at Science was the fact that we could get very high sieving coefficients in the neighborhood of the cut-off. For 5nm gold the sieving coefficient was 0.8 (i.e. the filtrate was 80% as concentrated as the stock solution)…
In my last couple of posts with respect to endothelial cell culture on pnc-Si transwells, I presented images of cell clumps over the active pnc-Si membrane area of transwells (here and here). There were dead cells associated with these clumps and this is a concern for barrier function experiments. This post shows a counter-example of…
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OK. So the bare membranes are no good for desalting by dialysis at the levels one might encounter with ‘salted out’ protein preps.
Lets hope some of these new treatments help.