Similar Posts
Fluorescein transport: O-ring position and wafer variability
After diffusing through a polymeric or pnc-Si membrane in the transwell format, diffusing molecules meet an “unstirrred water layer = UWL” on the bottom of the membrane. The UWL acts as another resistance to transport into the receiver compartment. According to some PAMPA papers, the UWL can be greater than 1 mm in thickness without…
NSF STTR Goals
ByJimPhase II submission August 1, 2016 or January 30, 2017 UR Tasks – Biocompatibility by Feb. 1 2016 Channel design optimization by November 2015
Sepcon-transwell discoloration II
I repeated a previous experiment but this time I used samples (no RTP) from W685 in oven @ 37C in DMEM(-FBS). Four type of samples (blank chip with no slits, chip with both intact membranes, chip with one intact membrane and chip with both broken membranes) were used at different time points. Again the expected…
UV-Ozone was broken, now fixed?
Nakul and I noted last week that the UV-ozone system was not performing. We took pictures of control and treated samples (2x) to measure contact angles on Amorphous, pnc-Si, Oxide and Nitride TEM grids. Our results were pathetic – most samples had less than a 10 degree decrease in contact angle, staying near 60 degrees….
Thin Film Electrode Creation
I have been trying to fabricate my own Indium Tin Oxide (ITO) electrodes on coverslips for the BBB device in order to minimize the working distance for any microscopy. Currently, I am using microscope slides that are way too thick (mm). Coverslips are generally about 0.2mm thick. I am using a combination of sputter, photolithography,…
BREAKING NEWS! The technique of UV Ozone Bonding is now complete
ByHenryHi all, the technique of UV Ozone bonding (of PDMS to glass, Si or pnc-Si) is now complete, serendipitously. Hsin from Miller lab want to bond PDMS to glass, they tried plasma oxidation treatment and it worked. The method is as followed: plasma oxidation treatment for 35 sec (which did not bond the sample, when…
2 Comments
Comments are closed.
So vapor salinization slows degradation but does not prevent it and L15 is just fine.
1) We’ll need to find more complete surface chemistry to work in general media.
2) We still need to do the ‘reconstitution’ studies to prove that it is a combination of bicarbonate and salts that causes the trouble in DMEM and other media – lets do this next please.
Anant,
If you vapor silane treat the surfaces – but don’t oxidize them beforehand with peroxide – what do you think is happening?