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Zeta Potential measurements with MgF2 nanomembranes
I believe that we can use MgF2 nanomembranes as electroosmotic pumps, along the same lines as pnc-Si and NPN materials. To first get a sense of how much pumping I would expect, I wanted to know what the zeta potential of these membranes would be, as the potential is directly proportional to the amount of…
w705 Permeability and Separation
W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration in the centrifuge. PVP enabled flow in the pressure cell in an otherwise wet/dry configuration….
Separations with Millipore and Pall Membranes
In anticipation of the pnc-Si separation paper, I have performed a couple of preliminary separations using NanoSeps and MicroCons. I applied 200ul of 1:20 brain extract ( ~.9mg/mL) to 100k NanoSeps and MicroCons, and I spun the samples for 3 minutes at 14000g. I had wanted to collect the retentate as is from the top,…
More science from the CNT folks…
Just in case you missed this one: www.nature.com.ezp.lib.rochester.edu/nnano/journal/v2/n11/full/nnano.2007.354.html This article makes the the previous papers seem mundane. It has inspired me though – maybe we can create a computer simulation that will demonstrate that pnc-Si membranes will dance and sing songs under the correct conditions. I will leave the choice of genre to the programmer…
Thinkin’ Secretion in the µSiM
ByJimOne of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289 where he showed that stimulation of ECs with TNFα from the abluminal chamber caused an…
Thickness and uniformity of PDMS layers
The last few times the spinned PDMS layers were much more uniform. The first picture below is with 1:100 diluted PDMS layer coated on it and the second one is a clean sample from the same wafer for comparison. The PDMS layer on the first picture is approximately 60nm thick. The measured thikness produced by…
