Hemo-Dialysis Channel Flow Characterization
Dialysis Rate: 10 mL/min, Péclet Number: 1, Membrane Width: 700 µm, Channels per chip: 17 Péclet number Pe ∝ H Pe ∝ 1/L Pressure Drop, P P ∝ 1/H3 P ∝ L
-
-
Urea Assay Standard Curve
The urea assay is straight forward. 6 wells are filled with 0, 1, 2, 3, 4, and 5 nmol of the urea standard (50 nL). Each well is activated with 50 nL of the reaction mix. Reaction Mix Sample control Urea Assay Buffer 44µl 46µl OxiRed Probe 2µl 2µl Enzyme mix 2µl 2µl Developer 2µl…
-
Clearance, Kt/V, and Concentration in a Reverse Flow Dialyser
Instantaneous Clearance: Kd CBi, and CBo are the concentration of solute in the blood at the input and output of the dialyser. CDi and CDo are the concentration of solute in the dialysate at the input and output. QBi, QBo, QDi, and QDo are the flow rates at the inputs and outputs. The volume of…
-
Microfluidics in Hemodialysis Project
The purpose of the fluidics in our hemodialysis system is to move the sample to and the retentate away from the membranes. The dialysate (permeate, filtrate) will be handled passively in the initial designs and therefore do not require fluidic manipulation. The parameters influencing the movement of the sample past the membranes include channel geometry…