The urea assay is straight forward. 6 wells are filled with 0, 1, 2, 3, 4, and 5 nmol of the urea standard (50 nL). Each well is activated with 50 nL of the reaction mix.
| Reaction Mix |
Sample control | |
| Urea Assay Buffer | 44µl | 46µl |
| OxiRed Probe | 2µl | 2µl |
| Enzyme mix | 2µl | 2µl |
| Developer | 2µl | 2µl |
| Converter enzyme | 2µl | 0µl |
Four trials of the standard were run. The first two, my first assays of any kind, were run on one day, the second two on the next day.
I am planning to write a proposal to the NIST Measurement Science and Engineering (MSE) Research Grant Program. Federal Funding Opportunity (FFO) is here. The proposal is due in the beginning of June. From talking with a friend at RIT would has one of these presently, it sounds like the best strategy is to target an…
As a proof of principal experiment, we wanted to test if Kilean and I could extract RNA from exosomes following their capture on 1298 membranes. As noted in Figure 1, we first used ultrafiltration to capture exosomes on the nanoporous membrane. We then attempted exosome lysis and RNA release by flowing the lysate below the…
Attached is a paper I’d like to review on Thursday (June 11, 2015). This group is using two planar surfaces in an attempt to mimic a 3D cell culture environment. This is similar to our claims that cells growing on a permeable membrane can be akin to a slice of a 3D matrix. I met…
For this experiment, I looked into the stability of RTP treatment. Specifically, I tested discoloration rates for chips at different time points after I did RTP. The idea was to see if the beneficial effects of RTP depended on the time that chips were stored after RTP processing. Here’s the data: All chips were from…
I am having some problems with my electroosmosis device and I was hoping that people with more knowledge about the TEM chips might have some suggestions. For these trials, I used membranes from a wafer labeled 412-S. I am still using the PDMS device from my last post although now I have mini “clamps” that…
This data is from about a month ago and it something that Jim has been asking me to put up, so here it finally is. When Tejas runs his experiments, he usually leaves me the last mL of blood that he has from his draw in case I want to do anything with it. One…
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My thought is that a smaller continuously operated device may allow some patients to be put on dialysis earlier than with standard equipment. Since the device requires the same vascular access as traditional HD, is early application even feasible? Will early application of dialysis affect the progress of renal failure for some failure mechanisms?