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Variation of TEER as a function of membrane area (COMSOL simulations)
Background: This is a follow up to the blog that Jim wrote on the basics of TEER measurement. This is the link to my old blog, which also describes the theory and some other preliminary data about TEER measurements. The resistance (R) of a wire is the resistivity (ρ) times its length (L) divided the cross sectional area (A) of…
ITO Embedding
I recently bought some slides of ITO from Sigma-Aldrich, and have been working to integrate them into PDMS by using the same process as I did for the glass coverslips (seen here) The main differences between the glass coverslips and the ITO coated microscope slides are the ITO film, which seems to have different wetting…
The Enemy Within: Optimizing an OLCN PUA Coated Microporous Membrane Mimetic and Exposing an Unlikely Saboteur
The battle for soft membrane coatings wages on as we continue to optimize polyurethane acrylate (PUA) coatings for our osteomyelitis project, whose background is described here and here and initial progress is described here. Since our last update, we have honed in on our most promising recipe and tried to fine tune its settings in…
Bonding SiMPore Chips Directly to Glass has been Difficult
Our friends at McGill university have wanted to incorporate our chips directly into their platform for squeezing and confining DNA. Their desires come in a form of a device where we can seal the SiMPore chips into glass channels without any intermediate gasket layers. In the video above, we can see the relevant layers, a…
HUVEC Growth on Si
Over the past five days Anant and I monitored human umbilical vein endothelial cell (HUVEC) proliferation on three substrates: RTP-treated Si samples (both with and without membranes), glass (microscope coverslips), and plastic (6-well tissue culture plates). After the first two time points very few cells were observed on the silicon samples, so we added more…
Exosome Isolation Using Tangential Flow Update
ByKileanHey all! In this post, I’d like to present some of my final data on the exosome isolation project and cover some data that was generated in response to questions asked during our previous discussion. To begin with, Tom asked whether or not it was possible to measure protein in the filtrate and retentate and…