Below are the results of my recent crosslinking efforts. PEG with 1.5% crosslinker was exposed to UV light at varying times through a bar printer/transparency mask measuring 100/200. In theory this corresponds to 100 micron PEG bars and 200 micron openings. In practice the results are much less neat. The table shows HUVEC cell attachment…
We have had success cross-linking PEG to TEOS membranes pretreated with TPM to aid in adhesion. This was done first on nonporous membranes, and then porous. We used 50% PEG at 1.5% cross-linker, which cross-linked at 8000ms. For comparison, 100% PEG at 3% cross-linker cross-linked at 5500ms. Nonporous TEOS Bottom image is at 4x. The…
Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and DI or PBS. We are testing for what will make the most optimal cell culture…
A control was done to observe cell proliferation on a TPM coated cover glass. Images were taken at 10x after 4 days. TPM coated cover glass Polystyrene petri dish We are having some success with the seeding of bend.3 cells onto microarrays sterilized with UV exposure. These were allowed to grow for 4 days. Images were taken…
Updates: We can now put the glass on top of the mask, which allows the use of nonstick agents in the creation of PEG microarrays. The ability to cross-link PEG-Diacrylate (PEG-DA) with a photoinitator and UV exposure was previously tested and shown to work. At the lowest power setting on the Leica (~25%) fluorescence source, we were…