Here is the most up to date figure that I’ve made to compare the simulated and experimental separation results. I went back through the x-ray crystals and remeasured the longest distances in the molecules. I used the radii to rerun the simulations and plotted them against protein and rhodamine diffusion data. Here are the results:…
In this post I will explain how I tried to use the 1D diffusion model to check the results we had in the Nature paper. Unfortunately I didn’t get the same results in the simulation and I present some ideas for the discrepancy. In the Nature paper we made simple small volume diffusion chambers and…
In this post I will show that the analytical 1D model of diffusion is similar to our 3D COMSOL model, with the exception that the times do not seem to line up. This is reasonable though since the 1D model does not take into account the non-membrane silicon pieces and enlarged volumes that occur in…
Presentation jc_9_15_2009_maryna. Paper nano9_30_305304
A temperature series was performed last week over seven wafers annealed inside the susceptor (15 nm silicon). The objective was to determine if the current configuration of target materials would show pore tunability over the temperatures of interest (900 – 1100 C). Below is a series of TEM micrographs and their corresponding histograms. At the…
This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells. These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days. Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but one survived this 7-day cell culture experiment. These cells were plated on the bottom on…
In my previous post, I showed images of bEnd (P8) cells on PET and pnc-Si transwells. These were seeded at 105 cells/cm2 and grown for 14 days. This post contains the TEER data. I simply normalized all values to the Day 0 (no cells) resistance, so everything starts at 100%. This data for PET matches…
I continued to run some of the samples that I described in this post last week. These are P8 bEnd3 cells, seeded at 100000 cells/cm2 (just below confluence) and grown for 14 DAYS. The pnc-Si was SC126 that was RTP’ed. I measured the TEER of these samples for 2 weeks and then stained the cells…
Using the same procedure as in the last post, I was able to simulate a 6um thick membrane with the same pore characteristics as the experimental pnc-Si membranes. Material diffuses more slowly through this membrane, but not as slowly as I expected. The following figure shoes the filtrate/retentate concentration ratios at 24 hours for a…
Last week, we ran several wafers with a silicon target from Refining Systems (Las Vegas). In addition to the source material being different, the target bond was a silver elastomer vs. the typical indium bond that AJA uses. Below is a TEM micrograph of a comparison between the RS silicon and AJA silicon. Both these…