I used the source meter that Chris brought over and tested the voltage at points across the chamber. I set up the system with 1x PBS at 15V (~10mA) with a pinholed membrane. I tested the voltage drop at the outer edges of the well, in the center, and the inner edge of the chamber…
I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into the lower plastic of the device to connect a pbs resevoir on the slide to…
I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was too dark, as seen by the image below. Harold Smith gave me some Ponceau S…
Given the low burst pressures of recently produced wafers Chris requested I burst pressure test some samples in two different orientations: well side facing away from the positive pressure source and well side facing towards the pressure source. The standard protocol I follow is to test the samples with the well side facing the pressure…
Today I built a small scale electrophoretic cell using the components from Lingyun’s study. I drilled out his cell and affixed a coverslip to the bottom. I set up a pinholed membrane just like our standard diffusion assay (with silica beads and PBS underneath the membrane). Two platinum wires were used as electrodes with the…
Last week, five wafers were produced – three had a double 3500 A wet oxide growth/strip and two had a single 3500 A wet oxide growth/strip. From Jess’ AFM work, an as-is wafer showed a high density of 5-7 nm particles on the wafer surface. With a double oxide growth/strip, these particles do not show…
I oxidized 2 intact chips from w639 using the following protocol: 10min with secondary generator, 15min with both primary and secondary generators. This protocol is slightly different than my normal protocol (10min 2nd, 5min 1st+2nd, 10min 2nd) because I messed up the settings, although the current protocol is arbitrary anyhow since we haven’t tested it…
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which show flux an order of magnitude greater than cellulose membranes. Our next step is to…
I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell. In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621. After that I first tried to see if DI H20 would also work (also pin. At 15V (which…
JP handed me a stack of wafers today, and I went over to the Krauss Lab and with the help of Jack Calcines got some AFM images. I didn’t have much time to play with, so I tried to get the most data I could quickly. I think we’ll have to do a more exhaustive…