Manufacturer’s Data Delivery Date: 5/20/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: NPN 1 Slot SKU: NPSN100-1L Lot Number: 1540 Quantity Received: 225 Manufacturer Test Report: Pore Size: N/A Porosity: N/A Flow Data Avg in SLPM 2psi: N/A 4psi: N/A Burst: N/A Packing slip & invoice: None UR Testing Porosity: 9.9 +/- 0.8%…
The purpose of this study was to characterize pore sizes on Aquamarijn (Aquamarijn Membranes B.V., the Netherlands) membrane chips. Membranes provided to us by Aquamarijn have a design specification of 450 nm pore size across 14 windows in the chip. Chips were sputter coated with gold particles at 20 mA for ~60s after which SEM…
In Part 1 we explained that the composite barrier in our model of the post-capillary NVU is determined by RBBB = RBlB + R2 + RBrB where is R2 is the resistance of the ‘perivascular space’ in our chip. Unlike the other two, the perivascular space is not a regulated cellular barrier that we need…
The SepCon filtration devices represent an innovative housing solution for Silicon Nitride membranes utilized in the filtration of microparticles and nanoparticles. In assessing compatibility, I conducted a test to evaluate the suitability of Aquamarijn’s microsieve membranes with our existing SepCon devices used in microplastic research. In the initial experiment, a standard SepCon assembly and filtration…
Introduction The modular μSiM is based on a 96-well plate format with a 100 μl open well. While these devices have proven extremely useful for various barrier models including the blood-brain barrier1 and the tendon-vascular interface2, there is a need to transform the open well into a fluidic channel. A closed channel device would enable…
Introduction Alzheimer’s disease (AD) is a neurodegenerative disorder that is characterized by accumulation of amyloid beta (Aβ) peptides and neurofibrillary tangles (NFTs) made of aggregates of the protein tau (1,2). It results in the destruction of neurons and their connections due to this accumulation of amyloid beta and tau (3). Tau is a microtubule associated…
Introduction The Slide Body (Figure 1) has been used for a long time to image μSiM devices. This slide body has the same footprint as a traditional microscope slide but is designed to hold up to 2 μSim devices for imaging. The device has an open center with thin ledges where the edges of the…
Introduction Sepsis refers to an uncontrolled systemic inflammatory response which can result from bacterial blood infections. We have long been interested in developing a sepsis model using the μSiM-BBB platform because the neuroinflammation stemming from sepsis can cause severe BBB disruption. For the majority of our stimulation experiments we have relied on cytomix, a cocktail…
The two barrier anatomy of the post-capillary NVU and why it matters At the post-capilliary venule, the blood-brain barrier (BBB) is actually two barriers in series: 1) one created by the pericyte/BMEC layer at the blood interface and 2) one created by astrocytes and the astrocyte-produced glia limitans at the interface with the brain. We’ll…
Dave Fang posted a poster in 2010 about particles found in DI water. I encountered the same issue during the nano-pocket permeability experiment. He said, “I’ve become increasingly suspicious of membrane clogging during the … permeability experiments.” I conducted an experiment with ultrapure water (UPW), typically filling a 1L glass container from a UPW machine….