Towards Surface Enhanced Raman Functionalization of Nanopocket membranes Introduction Use of Raman microscopy is used to evaluate the chemical profile of substances. Surface enhanced Raman spectroscopy (SERS) is a signal-boosting techniques where the weak Raman signal is amplified through plasmon interactions at a metalized (often nanostructured) surface. SERS for extracellular vesicles (EVs) was recently…
Introduction The Ehler-Danlos Syndromes are a group of thirteen heritable connective tissue disorders. These disorders are genetic, and usually involve a mutation in genes that code for components in the collagen matrix (1). Some of the main characteristics in patients with these disorders include tissue fragility, joint hypermobility, and skin hyperextensibility (1). Classical EDS (cEDS),…
The following site may be useful to draw to people’s attention when specifying objectives on the Etaluma: https://www.microscope.healthcare.nikon.com/microtools/resolution-calculator/#Focus I have configured a custom resolution calculation using the sensor data supplied by Etaluma, here exemplified at 10x/0.3NA: To take account of the shorter tube lens (which gives ¼ magnification expected cf Nikon 200mm tube) I have…
Manufacturer’s Data Delivery Date: 2/28/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: NPN 1 Slot SKU: NPSN100-1L-TE Lot Number: 1526 Quantity Received: 211 Manufacturer Test Report: Pore Size: 49.2nm Porosity: 15.5% Flow Data Avg in SLPM 2psi: 0.130 4psi: 0.248 Burst: 5.510 Packing slip & invoice: None UR Testing Porosity: N/A Pore size:…
Manufacturer’s Data Delivery Date: 2/28/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: NPN 1 Slot SKU: NPSN100-1L-TE Lot Number: 1523 Quantity Received: 237 Manufacturer Test Report: Pore Size: 52.7nm Porosity: 15.0% Flow Data Avg in SLPM 2psi: 0.126 4psi: 0.276 Burst: 6.151 Packing slip & invoice: None UR Testing Porosity: 13.7 +/- 0.5%…
Introduction This post will describe the preliminary work I have completed using size exclusion chromatography (SEC) for EV enrichment. We are ultimately interested in interrogating serum EV biomarkers using CAD-LB, however, the presence of protein and lipoprotein contaminants reduces the capacity for EV capture (~104) on NPN membranes and may hinder the analysis of rare…
Introduction This post provides an update on a series of experiments conducted with Sam. These experiments were designed to assess the versatility of the CAD-LB platform for detecting EVs in urine samples. Urine is a complex biofluid often used in clinical diagnosis, making it a suitable subject for testing the CAD-LB’s biomarker detection capabilities. After…
For year one of the Trace grant, I will be focusing on the development of a standard operating procedure for the uSiM-CA. To begin this process, I am transferring all of my current operating procedures from the Andor dragonfly confocal scope to the new Etaluma microscope. The Etaluma scope will be automated, collecting images of…
Introduction: Developing innovative therapeutics and treatment approaches with improved effectiveness and safety necessitates the use of cell co-culture models and barrier environments that mimic in vivo conditions. These models involve culturing two or more cell types together in vitro, allowing researchers to study cellular interactions, signaling pathways, and responses that closely resemble those seen in…
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