George Wadsworth Training Visit

Pnc-Si membranes have a high rate of EO pumping, but what is the best way to compare to other pumps?  The initial figure we used is an updated version of a figure found in a paper about alumina EO pumps (original figure bottom of this post).  In this figure we show flow rate normalized to…

Below is an image of the PDMS array on the nitrocellulose membrane. I’m trying to create a stamp that can be applied to the NC membrane, before PDMS is poured on. The idea is that the stamp will be heavy enough to keep the PDMS from soaking into the areas of the NC that the…

Membrane pore spacing can modulate endothelial cell- substrate and cell-cell interactions (REVISION) We submitted our “focal adhesions” paper to ACS Biomaterials Science and Engineering late last year. We received reasonable reviewer requests from three reviewers. I think I am mostly finished with revising the figures. Here are the updated figures and my comments on what changed….

I decided to find another supplier for HBSS and found one: CellGro.  CellGro HBSS had the same components as Gibco HBSS, although a couple of the concentrations were different.  I tested out this new HBSS vs. homemade HBSS. Here, the commercial versions of HBSS caused discoloration within a day, but my homemade HBSS didn’t. The…

This is a retrial of the negative adsorption assay using the Tecan spec/fluorimeter that we have been demo-ing. To perform this assay, I am applying a known concentration to the surface I want to test. After an hour I remove some of the solution and measuring the remaining concentration using absorption and the Bradford assay….