Introduction We are interested in exploring whether certain types of extracellular vesicles (EVs) may be more likely to become trapped while crossing the blood-brain barrier (BBB). Specifically, we are curious if EVs possessing certain types of surface integrins might bind to the vascular basement membrane that surrounds the exterior of brain blood vessels to become…
Introduction As what we’ve learned from the previous “Catch-and-Display Immunoassay” (CAD-IA) trials, we switched to an antibody pair from OriGene with low non-specific binding ability to each other and start to conduct ultracentrifugation for all reactive reagents for the reduction of protein aggregations. However, we found an inconsistency of co-localization percentage of immunocomplexes and beads…
Manufacturer’s Data Delivery Date: 4/5/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: 0.5-micron microslit SKU: MSSN400-3L-0.5 Lot Number: 5150 Quantity Received: 180 Manufacturer Test Report: Calculated Specs: Porosity: ~10.1% Slits/Membrane: 6820 Slit Length: 50um Measured Specs: Avg. Slit Width: 0.47um Avg. Flow @ 2 PSI: 1.142 SLM Avg. Burst Pressure: 8.2 PSI Packing…
Author: Kevin Ling Dysfunction of the outer retinal blood barrier (oRBB), which is composed of the retinal pigment epithelium (RPE), Bruch’s membrane, and choriocapillaris (CC), is implicated in age-related macular degeneration (AMD), which causes irreversible central vision loss [1-4]. AMD is the leading cause of blindness in industrialized countries; it is estimated that by 2040,…
Manufacturer’s Data Delivery Date: 3/26/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: 2 Slot NPN – Wide SKU: NPSN100-2LWMS Lot Number: 1537 Quantity Received: 300 Manufacturer Test Report: Avg flux SLPM at 2PSI: 0.197 Avg flux SLPM at 4PSI: 0.374 Avg Burst Pressure: 4.589 Average porosity: 11.8% Avg pore size: 48.1nm Packing slip…
Manufacturer’s Data Delivery Date: 3/14/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: 8-micron microslit SKU: MSSN400-3L-8.0 Lot Number: 5203 Quantity Received: 102 Manufacturer Test Report: Nano Pore Height: 8.022um Avg flux SLPM at 1PSI: 3.272 Avg flux SLPM at 2PSI: 4.465 Avg flux SLPM at 4PSI: 7.185 Avg Burst Pressure: 17.513 Packing slip…
Manufacturer’s Data Delivery Date: 3/14/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: NPN 3 micron Dual Scale High Porosity SKU: DSSN100-1L-10%-3.0 Lot Number: 1531 Quantity Received: 315 Manufacturer Test Report: Avg Porosity: 15.1% Avg Nano Pore diameter:50.7nm Avg Micro Pore diameter: 2.986um Avg flux SLPM at 1PSI: 0.445 Avg flux SLPM at 2PSI:…
Manufacturer’s Data Delivery Date: 3/14/2024 (picked up and logged by Ahmet Gurcan) Membrane Type: 2 Slot NPN – Wide SKU: NPSN100-2LWMS Lot Number: 1532 Quantity Received: 299 Manufacturer Test Report: Avg Porosity: 13.4% Avg Pore diameter: 48.7nm Avg flux SLPM at 2PSI: 0.244 Avg flux SLPM at 4PSI: 0.462 Avg Burst Pressure: 6.564 Packing slip…
The μSiM Vasc-SkM Microphysiological development can be separated into 4 parts: Media tuning for integrated culture Force sensing element setup Assembly of the μSiM Vasc-SkM and setting up the perfusion Transendothelial Electrical Resistance (TEER) measurement of the Vast component Work has been done on the first 2 parts. Media tuning for integrated culture In order…
Introduction: In the previous post, we demonstrated our capability to fabricate the nano-pocket membrane and effectively capture and release particles of various sizes. In this post, our focus shifts to exploring the membrane capability. We will focus on the membrane modeling (parking point, the plateau region)of the nano-pocket membrane. To have an overview of the…