Harold Smith at the Med Center proposed an experiment for Jess and I to try, in which we use our membranes to separate proteins onto a PES, PVDF (Polyvinylidene fluoride) or Nitrocellulose membrane, similar to a Western Blot of DNA microarray. The idea is to create a system to screen for drugs that break protein-protein…
Previously I ran a chemical stability study of samples from W613, which has a 30 nm thick pnc-Si layer. In that study complete failure of untreated samples was observed by 18 hours in cell culture, which was not a significant improvement from sample lifetime of 15nm thick pnc-Si. To see if RTP treatment helped the…
Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
Based on the results obtained yesterday with the closed flow cell we reran another trial today. The closed flow system we tested today was bound using UV Ozone treatment, surprisingly this system created a relatively strong bond despite our resent results. Once the UV Ozone treatment was complete and the system had bound vacuum grease…
I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs. Fibroblast spreading on glass 1 Fibroblast spreading on glass 2 Time lapse movies for fibroblasts spreading on pncSi…
We’ve been assuming that porosity follows a linear relationship with respect to wafer position, but this turns out to be incorrect. I imaged membranes from five points on wafer 620 and found that maximum porosity does not occur at the outer most sample, as previously thought, but instead at r=3. Does this provide a better…
I mixed made solutions of PBS and Rhodamine with varying Rhodamine concentrations and ran them through the spectrometer. Below is the concentration vs absorption curve I made from it. I blanked the spec with PBS instead of ddH2O since the solution is in PBS. The slope is almost double for Rhodamine in ddH2O compared to…
Yesterday Dean, the student from RIT, came over to help us set up our Anodic Bonder. Once we get the proper leads and connections we will be able to perform these bonding trials whenever we want without needing to travel over to RIT. In the meantime we have continued our work with PDMS, and our…
Here is the full updated Adsorption Quantification figure: We were concerned that cellulose appeared to adsorb more than PES when the literature clearly states that it shouldn’t due to its hydrophilicity. We considered that this same hydrophilicity might be part of the problem with our method. Macroscopically, it appears that the protein solution applied to…
Spreading of cells (huvec and fibroblasts) on pncSi and glass was observed at ~37 C in L15 media (+ 10% FBS) for ~5 hours. Its observed that the cell spreading is pretty similar on both the substrates and the time lapse movies can be seen from the following links: huvec_glass_sprd huvec_membrane_sprd fibroblast_glass_sprd fibroblast_membrane_sprd Somehow its…