Electro-osmosis: Yesterday I tried to study the electro-osmosis-like phenomenon that we were observing with the electrophoretic cell. All membranes again had pinholes. I set up the wells as before, but I would remove 50ul from the well which the negative electrode was attached. I turned on the voltage and waited until the water level came…
Last week, Lingyun and I deposited 30nm SiO2 onto both sides of wafers 335, 341 and 345. Unfortunately, these wafers weren’t in too good shape, so there are only a few intact slits without pinholes. I then took a few chips from each wafer and did RTP (800C, 5 min, Ar). I’m doing discoloration studies…
I recently repeated a chemical stability test of RTPed (5 min in Ar at 800C) samples from W613, which has the 30 nm pnc-Si layer. In this study I used DMEM +10% FBS as the culture media without cells. Previously RTP treatment of samples from this wafer survived about 7 days. In this iteration of…
I finished the first experiment to look at rhodamine transport across commercial transwell membranes. I was mainly interested if there would be enough transport into the basolateral chamber to measure the rhodamine with the spec. Experimental details: both chambers in PBS and at room temperature, spinning on the rotomix, 0.5mM initial rhodamine in donor; sample…
We were worried that Friday’s experiments were giving us a false positive results. If the electrophoresis breaks the membrane, then we’d expect to see the rhodamine rush through just like it was in our experiments. It’s too hard to view the membranes afterward because they tend to break during removal. I designed some simple tests…
Last week I took a series of TEM images from all but one position on wafer 638. Below are the TEMs, histograms, and porosities vs. distance from center plots. “Xpos”, “xneg”, “ypos”, “yneg” indicate which axis the images were taken from. Positive x-axis Negative x-axis Positive y-axis Negative y-axis Wafer 638 porosity vs. position While…
Because of Harold Smith’s project, Rachel and I have been working on various methods to get proteins through a pnc-Si membrane and onto a polymer membrane. The particular system that Dr. Smith wants to study is very sensitive to ambient RNAses, molecules that digest RNA. This means that the long times required for a diffusion…
I’ve been learning the discoloration protocol from Karl and Anant and finally have some pictures of my own to show. We got a couple of new media (RPMI and DMEM/F12) last week in order to grow the cells I’m getting from the Elder lab (rat lung cells). I did a discoloration test to see if…
Today Barrett and I processed SiO2 deposition at RIT. This process was actually delayed due to the cleanroom scrubber failure last week. We deposited 30nm SiO2 on both sides of three wafers, #335, 341, and 345. Earlier in March, I tried 20nm and 40nm SiO2. It was found that 40nm SiO2 is so thick that…
One question we recently had was what does it look like when we perform a separation experiment when a membrane has a pinhole. I selected two membranes off 621 that appeared to have pinholes on both slits. I applied 1:2 standards to the top and allowed to diffuse for 24 hours. I collected the retentate…