Today I built a small scale electrophoretic cell using the components from Lingyun’s study. I drilled out his cell and affixed a coverslip to the bottom. I set up a pinholed membrane just like our standard diffusion assay (with silica beads and PBS underneath the membrane). Two platinum wires were used as electrodes with the…
Last week, five wafers were produced – three had a double 3500 A wet oxide growth/strip and two had a single 3500 A wet oxide growth/strip. From Jess’ AFM work, an as-is wafer showed a high density of 5-7 nm particles on the wafer surface. With a double oxide growth/strip, these particles do not show…
I oxidized 2 intact chips from w639 using the following protocol: 10min with secondary generator, 15min with both primary and secondary generators. This protocol is slightly different than my normal protocol (10min 2nd, 5min 1st+2nd, 10min 2nd) because I messed up the settings, although the current protocol is arbitrary anyhow since we haven’t tested it…
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which show flux an order of magnitude greater than cellulose membranes. Our next step is to…
I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell. In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621. After that I first tried to see if DI H20 would also work (also pin. At 15V (which…
JP handed me a stack of wafers today, and I went over to the Krauss Lab and with the help of Jack Calcines got some AFM images. I didn’t have much time to play with, so I tried to get the most data I could quickly. I think we’ll have to do a more exhaustive…
We received W648 from the production team this week. Upon inspection of this wafer, is has a higher density of pinholes than we have been seeing lately (>3 pinholes/slit compared to 0 or 1/slit) and seems to show a return of contamination, shown below. This contamination is visible over the entire wafer, but is easiest…
In this week’s meeting there was a request to have Maryna and I make our BP and AP data available online. While Maryna is at the Gordon Conference I thought I would upload what I have. The attached spreadsheet contains two tabs, one for AP and BP. Wafer number and position of each test sample…
Motivated by Tom’s suggestion at lab meeting on Tuesday, I tried “conditioning” the DMEM+10%FBS before adding the chips. “Conditioning” just means that I allowed the DMEM+10%FBS to equilibrate in the incubator for an hour before adding the chips to test if a brief (~1 hour) exposure to very high pH causes discoloration. DMEM+10%FBS without the…
There is AFM images that were taken in Krauss’s lab before I left. I wasn’t able to see open pores. There were samples from two wafers (213 and 398) with deposited polymer. On samples from first wafer, the polyimide deposited very well and surface is smooth. On w398 the polyimide layer has hills which are…