Below is an image of the PDMS array on the nitrocellulose membrane. I’m trying to create a stamp that can be applied to the NC membrane, before PDMS is poured on. The idea is that the stamp will be heavy enough to keep the PDMS from soaking into the areas of the NC that the…
Last week in lab meeting, someone wondered if there the time after RTP affects the rate of discoloration. To test this, I RTP’ed chips from w638 in Ar at 800C for 5 minutes. I then followed discoloration in DMEM+10%FBS in the incubator with untreated w638 chips as controls. All of the chips discolored within a…
Last week, I did a couple experiments based on a suggestion by Tom. The pH of DMEM starts out quite high and then buffers down into physiologic range after some time in the incubator. Tom surmised that a short exposure of the chips to high DMEM pH might hasten discoloration. To test this out, I…
Hi all, For quite some times we have been trying to design a diffusion-based chemotaxis chamber that operates under an environment of 37 degrees Celsius in temperature. One main obstacle that we face is the evaporation of the 2 uL of rhodamine 6 G (R6G) that we load on the well openning of the membrane…
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which show flux an order of magnitude greater than cellulose membranes. Our next step is to…
Hi all, After experimenting with UV ozone bonding of PDMS to glass, Si, and pnc-Si for the past few weeks with Mike, we have reached the following conclusions: 1. The bonding of PDMS to glass is always stronger than the bonding of PDMS to Si and pnc-Si, given that all receive same treatment of UV…
I used the source meter that Chris brought over and tested the voltage at points across the chamber. I set up the system with 1x PBS at 15V (~10mA) with a pinholed membrane. I tested the voltage drop at the outer edges of the well, in the center, and the inner edge of the chamber…
I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into the lower plastic of the device to connect a pbs resevoir on the slide to…
I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was too dark, as seen by the image below. Harold Smith gave me some Ponceau S…
Given the low burst pressures of recently produced wafers Chris requested I burst pressure test some samples in two different orientations: well side facing away from the positive pressure source and well side facing towards the pressure source. The standard protocol I follow is to test the samples with the well side facing the pressure…