Last week, JP deposited 25nm SiO2 for me on wafer 644 (which I cleaned in the UV/Ozone before I gave it to him). Unfortunately, someone broke the E-Beam that day and JP was only able to deposit on 1 side of the wafer (the solid side). I did some experiments on the wafer anyway. First,…

Jamie asked me to post these images of our membranes taken by Dr. Xiao Zhang from Hitachi.  They used a 300 kV tool to acquire these micrographs. The spacing between the atoms (striations) tells us the crystallographic orientation; most of the nanocrystals we see are in a (111) orientation.  It’s interesting that the perimeter of…

Here are the results of a protein ladder separation with w677.  If you recall, 677 is the 200um wafer that did not fit into the SepCons but had very few pinholes. S = Standards Ladder, C = Protein solution that never came in contact with membrane, R = Retentate, F = Filtrate Myosin (Band at…

JMP7 statistical software was used to perform a Tukey-Kramer analysis on both burst pressure (psi) and air permeability for membranes fabricated on 200um and 400um wafers. This data has been collected from a spreadsheet that is maintained by Karl and Maryna. The following plots are only examples since there are several other conditions that may…

I talked about this yesterday in lab meeting, but here is the data.  Since the components of HBSS by themselves did not cause discoloration (HBSS components), I adjusted the concentrations of NaCl, KCl, glucose, Na2HPO4 and NaHCO3 to match that of DMEM.  The control was a wafer in DMEM/F12 with 10% FBS.  None of these…

Does more active area mean more fluid transported by electroosmosis? To test this I found an ancient wafer (w13) that had different active area geometries. Can someone check and make sure I got these right: Long slit – .1 x 1mm Short slit – .1 x .5mm Point – .1 x .1mm 3×3 Point Array…

An initial experiment for membrane deflection characterization using an optical profiler (Veeco Wyco) has been performed. Below are some of the images taken with the tool: (a image was taken at each 0.5 psi interval): Once we get a pressure regulator we will be able to generate numerical data of membrane strength/deflection down to 5.0…

Jamie and I met with Don Black with an electron microscopist that consults for NaturalNano. In efforts to test out our TEM window grids, he gave us halloysite nanotubes. These are naturally occuring and much larger than the carbon nanotubes we normally talk about. The samples were prepared in 0.5% Triton X-100 in water and…

This is a retrial of the previously outline negative adsorption assay.  I was a little more careful with cuvettes this time around (there are very significant differences in the plastic cuvettes) and I spread out the same amount of solution over a larger area.  If exposed to more surface, more protein should adsorb and leave…