This is a retrial of the negative adsorption assay using the Tecan spec/fluorimeter that we have been demo-ing. To perform this assay, I am applying a known concentration to the surface I want to test. After an hour I remove some of the solution and measuring the remaining concentration using absorption and the Bradford assay….
Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th. Please come try out your assays on it. Or check sensitivities. Jess or I could probably steer you in the right direction as far as the software is concerned. My impressions…
I attached a presentation here for tomorrow’s meeting. sensor
I decided to find another supplier for HBSS and found one: CellGro. CellGro HBSS had the same components as Gibco HBSS, although a couple of the concentrations were different. I tested out this new HBSS vs. homemade HBSS. Here, the commercial versions of HBSS caused discoloration within a day, but my homemade HBSS didn’t. The…
Last week, we wondered whether the wells of pncSi chips created an unstirred volume in which an extreme environment could form (pH, byproducts), thus accelerating discoloration. To investigate this, I placed one chip flat on the bottom of a plate and another chip balanced upright on the sidewall of a well. I rotated the plate…
I sealed track etched membranes into the usual E-Cell setup. Here’s a chart with the calculated flow rates: I’m not sure why the 50nm sample was so much higher. Maybe there’s a difference in porosity? Also this is only the first try at this experiment. Here’s a chart with flow rates of all the experiments…
What has been accomplished thus far: Able to detect BSA on NC membrane with the use of Ponceau S stain With PDMS array on NC, no BSA leaks through the area covered by PDMS Initial trial done with 10:1 ratio of elastomer base: curing agent. Later 3:1 and 5:1 ratios done. 3:1 is too sticky…
In these discoloration tests, I looked at the components of HBSS. HBSS consists of NaCl, KCl, 0.048 mg/mL Na2HPO4, 0.06 mg/mL KH2PO4, glucose and 0.35 mg/mL NaHCO3. In a previous post, I showed that NaCl, KCl and glucose didn’t cause discoloration (previous post). So, I made HBSS from scratch and I combined the various phosphate…
For this experiment, I looked into the stability of RTP treatment. Specifically, I tested discoloration rates for chips at different time points after I did RTP. The idea was to see if the beneficial effects of RTP depended on the time that chips were stored after RTP processing. Here’s the data: All chips were from…
We have two cutoffs for the Millipore Biomax PES membranes: 50kD and 30kD. I tested the “EO” rates for both of these membranes and plotted them in this figure with standard deviations (3 trials of 50kD and 4 of 30kD). 50kD has a significantly higher rate of “electro-osmosis.” I will try to compare these rates…