I mostly finished re-processing the images with new method of using pore processor. The images of molar flow/porosity dependence with older and newer processed data are below. Older image: New image: They don’t look much different. Also you can not compare them point by point because the porosity is different for both images after re-processing….
In yesterday’s post I showed that I was able to obtain good negative adsorption assay results, but there was one major problem. The cellulose and PES actually had higher protein concentrations after the assay. I suggested this was because they are drawing in water to some extent, but protein was not able to pass through…
The Tecan Nanoquant plate is amazing. For those of you who are unaware, we were able to demo a quartz plate that enabled absorbance measurements with only 2ul drops. It took a little bit for me to figure it out, but it really is a great tool for this kind of study. The ultra low…
I did a quick experiment in order to show everyone what the Tecan allows us to do. I made a dilution curve of rhodamine 6G in PBS and measured the absorbance and fluorescence with the fluorimeter. I first did an absorbance scan and found that the maximum absorbance was at 530nm (panel A). Then I…
Creating an array to hold a pnc-Si wafer to proving to be difficult. The PDMS spreads too rapidly though the NC to form a consistent array for the pnc-Si membrane (and eventually wafer) to rest on. I’m still running different trials of the PDMS on NC to see if I can get the array to…
Last week, we created a series of wafers that were RTPed at four different temperatures between 900 C and 1000 C. Below is a comparison of the 900 C (left) vs. 1000 C RTP (right) wafer. We see that a difference of 100 C shifts the cutoff ~10 nm. The pores are also the roundest…
Here is the current method that I am following to find the pore statistics: 1. Select the highest threshold that you can still close with disk of 3 2. Hit majority 3. Close w/ 3 4. — Try open w/ 3 5. Erode w/ 3 6. Dilate w/2 7. Dilate w/ 2 8. Erode w/…
W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration in the centrifuge. PVP enabled flow in the pressure cell in an otherwise wet/dry configuration….
We’ve been focusing on bicarbonate ions as one of the causes of discoloration. So, I made several sodium bicarbonate solutions and tracked discoloration of non-RTP’ed samples. I made 3.7 mg/mL NaHCO3 (=44mM, this is the concentration in DMEM), 1.85 mg/mL NaHCO3, 0.925 mg/mL NaHCO3, 0.4625 mg/mL NaHCO3 and 0.23125 mg/mL NaHCO3. I also ordered a…
The last couple of posts showed that homemade HBSS does not cause discoloration, even though commercial HBSS does. I wanted to test if there was something mysteriously special about this solution, so I looked at non-RTP’ed wafer 622 in the incubator in solutions with and without serum (10% FBS). Even without serum, the cell media…