Samples are from wafers #316 and 318. This is the first time for wafer scale deposition. Processing conditions:1) chamber base pressure @ 6×10-7 Torr (previously @ 5×10-6 Torr)2) 15nm Ti + 15nm Au, no vacuum break Estimated “cell resistance”: about 20-25 ohm, much lower than previous measurement. Possible reasons why lower resistance is observed:1) “Au…

I few weeks ago I posted some flow rate data based on the 100K microcons in which three of the four membranes worked fine and the other appeared to be a “dud”. I pulled the four systems apart and used the digital calipers to measure the membrane thicknesses and found the following. – The three…

The new smaller EQ setup that Tom came up with were tested in these experiments. The benefit is that there is much less dilution of our filtrates. 500ul of our sample (in these cases we tested 1.0 mg/mL and 0.5 mg/mL brain extract) is loaded into the SepCon and 250uL of buffer fills the reservoir…

First Try is detailed in this post. Three membranes from w334 were used to separate depleted serum. As before we see that all of the proteins pass through the membrane, and again we can observe the one middle band that seems to appear more in the filtrate (red arrow). It’s possible that most of these…

click here for the powerpoint presentation click here for the PDF presentation

This gel shows the separation using two 100k Microcon membranes and 1mg/mL brain extract. 500uL of 1mg/mL brain extract was loaded into the Microcon and spun at 14000g for 5min. 400uL passed through the membrane (100ul remained on top). It appears that their “cutoff” as we would consider it is much lower, more like 50kD….

Molar flow thought pores with different diameters under 1 psi. First, I calculated molar flow based on porosity histograms extracted from TEM images. And then compared it with the actual experimental data. Here are few examples of this. molar%20flow.ppt

Six wafers (385-390) were produced last week with the new Solaris 150 RTP system. Good news: We now have much better control/feedback compared to the RIT setup. There are two thermlecouples on the wafer tray that monitors the front and back portion of the wafer. The new unit allows us to independently modify the front/back/bottom…

This is a side view schematic of the experiments I’m setting up in Mike and Derek’s stir cell. The membrane separates two wells. In the well where the membrane is flush with the wafer I add 300uL 5nm gold in H2O. On the other side I add my buffer and a stir bar. Adding a…

The coating was done last week at RIT (it was postponed from two weeks ago because of the failure of the e-beam crystal sensor). I deposited 10nm Ti and 15nm Au, without vacuum breaking. All the samples are from wafer #313. TEM images were taken yesterday with the help of Karen. (Her machine is really…