A droplet (3uL) of 0.117 uM actin solution was added on membrane W303(0,2). Let the membrane sit in humidity Chamber for 20 min. Remove excess liquid. Add equal volume of Uranyl Acetate on membrane. Wait 90 sec. Remove excess liquid. Image was taken right after sample preparation. Xiao

I am trying to build a helpful spreadsheet that outlines all the experiments perfomed with a particular wafer. It also lists the conditions and quality of the wafer. Any other suggestions would be helpful. To view the spreadsheet, click here.

Hey everyone, thanks for helping me out with my presentation this morning. I posted A LINK to the ppt. below. Let me know if you have any questions or comments. Thanks! Counterflow Dialysis

Air permeability (w341, 342, 344, 345) Burst Pressures (w341, 342)

This is preliminary data for air permeability in track-etched membranes for membranes with different pore radius.For comparison here is a table of the performance characteristics for TE membranes Measured data is of the same order as official TE membranes data.

I’m currently trying to compare separations between different complex protein mixture. This was my first crack at depleted human serum. This mixture has been processed so that the most common blood proteins (such as albumin and immunoglobulins) have been removed. All three trials were performed using membranes from w338 in diffusion mode. 338 has a…

I measured the I-V characteristics of our coated membranes on Wed. at RIT. JP observed the measurement. The semicon parameter analyzer in the test lab is “old” but isexactly what we need! Roughly speaking, our coated membraneshave resistance about 2k Ohm, depending on the thicknesses ofmetal films (this is based on pure Ti coated samples,…

Last week’s production yielded six wafers, all deposited at room temperature. Here were the annealing conditions: Wafer 334, 343 – RTP @ 975CWafer 341, 344 – RTP @ 975C + FA @ 1000CWafer 342, 345 – FA @ 1000 C Pinhole density was moderate along the edges of the wafer, with one or two pinholes…

I used the EQ format to test the 100K microcons in a wet-wet trial using DI water. The below illustrates a comparison between the hydraulic permeability of the 100K microcons in a wet-dry format (from tests earlier today) vs. a wet-wet format. The group on the left (1) represents the wet-dry tests done earlier today…

I reran the 100K Microcon membranes using bovine albumin. This time I ran a set of four fresh membranes with DI water for a preliminary trial. Once complete I used two of the same four membranes with a 1mg/mL solution of bovine albumin in PBS to test the effects of protein; the other two membranes…