100K Microcon with Bovine Albumin: retest

I reran the 100K Microcon membranes using bovine albumin. This time I ran a set of four fresh membranes with DI water for a preliminary trial. Once complete I used two of the same four membranes with a 1mg/mL solution of bovine albumin in PBS to test the effects of protein; the other two membranes from the initial run where used as standards and rerun a second time using DI water.

The group on the left (1) represents the hydraulic permeability of the membranes when they were initially run with DI water (the third peak from the left is a “dud” most likely due to manufacturing) . The group on the left (2) represents the re-tests of the same four membranes from trial 1…
-the left two peaks of group 2 are those membranes run a second time using a 1mg/mL solution of bovine albumin in PBS
-the right two peaks of group 2 are those membranes run a second time using DI water; as previously mentioned the low peak is the same “dud” from trial 1.

These tests show more definitively that protein reduces the hydraulic permeability of the 100K Microcon membranes. Also we see again that running a membrane a second time immediately after running a first time with water results in an increased hydraulic permeability.