The permeability data is very different for films deposited at room temeperature and 400C.
The 400C deposited wafers have much higher permeability and more consistent.
w335 – RT
w336-RT
w338 -400C
w340 – 400C
Burst Pressures
A quick update since we last talked about how DNA controls were proving to be problematic: Things are working again (more or less). I went back top basics, remade most of my solution, ordered new DNA samples, and, most importantly, piranha-cleaned my chips. The first three experiments I did on basic 30-nm thick 5.4mm square…
Six wafers (385-390) were produced last week with the new Solaris 150 RTP system. Good news: We now have much better control/feedback compared to the RIT setup. There are two thermlecouples on the wafer tray that monitors the front and back portion of the wafer. The new unit allows us to independently modify the front/back/bottom…
Last week I performed spins with SepCons from w310. I tracked permeability and tried to perform some separations with proteins. w310 had high air permeability (link), but from my diffusion data it looked looked like the cutoff was possibly between beta-galactosidase and phosphorylase b (link). Myosin definitely did not make it through these membranes, but…
Katia in Dr. Shestopalov’s Lab functionalized some pnc-Si windowless chips. The process she follows is shown in Figure 1. She provided chips at various stages of the process. “Grignard”, “Diazirine”, and “PEG”. After functionalization, the chips were wetted with 20 µL of 1:1 pbs then 40 µL of 5 mg/mL f-BSA was added. The…
Introduction The glial fibrillary acidic protein (GFAP) is an intermediate protein released by astrocytes and has value as a serum-based biomarker of neurological abnormalities including traumatic brain injury1. Inspired by the CAD-LB method for digital assessment of biomarkers on small extracellular vesicles, we sought to create a digital immunoassay for the detection of protein biomarkers….
This is the third installment in a series of posts focused on the development of a method for measuring barrier permeability in the µSiM. In Part 1 we discussed the goal of making these measurements in situ at a position beneath the membrane. In Part 2 we validated this approach experimentally on the Andor Dragonfly…