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Particle Tracking in COMSOL Multiphysics
ByKileanThis is a continuation of my post from last time where I looked at modeling actual pore geometries in COMSOL. In this post, I explored the new feature that we have with COMSOL that is the Particle Tracing Module. This module really enables the visualization of particle behavior in our systems where we can better…
Fluorescence on Single-Well CytoVu – Phalloidin & DAPI
In this trial, bEnd.3 cells were seeded onto three single-well CytoVu devices with SiO2 membranes coated with a 1% Geltrex coating. After ~27 hours, the cells were fixed and permeabilized (the fixing may be only partial though, as the solution used was more than 5 months old). After this, DAPI and FITC-conjugated phalloidin were used…
Exploring primary human microvascular endothelial cells for μSiM-hBBB modeling
Introduction Sepsis is a maladaptive response to infection with a 25-50% mortality rate and a high incidence of cognitive decline among sepsis survivors.1-6 Because infection originates outside the brain, the factors responsible for cognitive decline in sepsis must get to the brain through the circulation and breach/act on the blood-brain barrier (BBB) to cause injury.7-9…
Urea/f-BSA/cytochrome c concentrations in retentate after dialysis: 12 hours
Continuing on the path of hemodialysis, we are increasing the complexity of the fluid being dialyzed. In this long-term experiment I will pump 100% serum with added urea (1mM) cytochrome c (1 mg/mL) and fluorescent BSA (1 mg/mL). So I need to detect urea, BSA, and cytochrome c in serum. Cytochrome c absorbs at 405…
W565 Air Permeability and Burst Pressure
I tested four samples (-1,1); (-1,2); (-1,3); and (-2,4) for air permeability and burst pressure. The first three samples showed no measurable air permeability for three attempts each. The sample from position (-2,4) did have a very small air permeability of 11.2 cc/(cm2*atm). Based on the results of rhodamine diffusion it seems odd that I…
Dye Diffuses through UV-Ozone Treated and Untreated SC267 Samples
ByMike HIn my last post I presented the results of an experiment in which it appeared that BSA did not diffuse through a SC267 sample treated in the UV-Ozone system for 20min at 150C. As a control I also ran BSA dissusion through an untreated SC267 sample and showed that BSA did move across the membrane….







