Fabrication of silicon membrane using polymer self-assembly template

I recently popped out membranes from wafers 1064 and 1065 for our lab. I only started with about half a wafer, but these are the numbers from the extraction: Wafer ID # of In-Tact Extracted Membranes : High/Low Porosity # I broke during extraction # already broken before extraction # apparently under etched 1065 (100W)…

After some experimentation with acetylene concentration and anneal temperature, I was able to find a carbonization recipe that appears to shrink our pores by 1/3 the original diameter.  Below are images of an untreated sample (TEM 148: 15 nm pnc-Si 1000 RTP) and a carbonized sample (see recipe at end of post). A closer look…

I’ve been attempting to simulate protein sieving using the COMSOL model.  This first figure shows the comparison of the simulated separations to the actual results as determined by gel densitometry. The simulated separations tend to lag behind the experimental data for smaller proteins.  This is surprising since the simulation is faster than experimental results for…

So I got few nanonitride chips with a pncSi cap on it from Josh (#1019, 320 um sub, 50 nm SiN, 40/20). I annealed them before using it for cell culture. Instead of using the existing CytoVu designs, I made some household cell culturing platforms as shown in the figure below. filled the whole petri…

Here is the excitation and emission wavelengths for two common fluorophores for transport: rhodamine and fluoroscein. rhodamine fluoroscein max ABS 480 nm 470 nm emission 558 nm 516 nm excitation 524 nm 492 nm The protocol for finding excitation and emission wavelengths of a fluorophore with use of the Tecan can be found here. During…

We’ve been moving to a new format for chips, trying to standardize an active area that has good yield and allows fluidic access through the chip itself. The SEPCON outer dimensions have been reused (5.4 mm x 5.4 mm). It’s a 1.5 mm^2 single slot chip area (2 mm x 0.7 mm) with vias that…