Similar Posts
Exosome Identification: Quantum Dot Labeling and NanoAuger Analysis
ByKileanAlright, so this is kind of a big update and I will probably split it into a couple of parts. Basically, in a search for a way of confirming that the particles we are seeing on the membranes, I have tried several techniques with varying degrees of success. The initial experiment involved me conjugating an…
Counter Flow System Progress
ByMike HJust wanted to post an update on the creation of a counter flow system using the pncSi chips from wafer 619 (the wafer with the etched trenches for the insertion of capillary tubing). Over the past few weeks Henry and I have been able to produce a few functional counter flow systems using UV Ozone…
Lab tours on Friday
ByJimProspective Graduate Students will be touring the lab this Friday from 12:30-1:00. Please be working in the lab and ready to engage!
Ozone-treated PDMS: Contact Angles
As seen in prior NRG post, ozone treated samples recover over about 24 hours. However, recent experiments at Gaborski Lab show high variability when using the Corona wand to treat samples with ozone. The goal of this experiment was to determine the contact angles of PDMS samples when exposed to ozone treatment. PDMS samples were…
Fibroblasts on pnc-Si transwells
One of the hidden secrets of endothelial angiogenesis assays on Matrigel is that non-endothelial cell types also form 2D capillary-like structures (tubes) on those gels. Motivated by this observation, I wanted to look at fibroblast behavior on pnc-Si transwells, specifically with respect to whether or not they formed vacuoles. Just to clarify – although Anant…
Flow Through Affinity Based Capture or a Lack Thereof, the Story of a Chemist’s Folly
Fluorescent Streptavidin Coated Bead Capture on Biotin KODE Coated Surfaces After determining that KODE molecules can successfully coat our silicon pore membranes, we set out to determine if the coating could be functional and how stable it was. To test both factors concurrently we designed experiments in an attempt to capture 1.24 um diameter fluorescent…
