Congrats Dave!

Introduction EC Apicobasal polarity in immunity has and will continue to be a theme of my thesis. I have presented on bits and pieces of this work over the past year or so, but now that this work is under review and more or less complete pending reviewer comments, I wanted to summarize our finds…

This paper (Variance of Streaming Potential) examines the sources of error in zetapotential measurements. Jess and Zydney both cite it in their EO papers as a source of experimental procedure – in particular this paper found that waiting for the pressure to equilibrate at discrete timepoints works better than a continuous ramp-up in pressure. From…

I finally got some MATLAB code working which calculates permeability coefficients (P, in cm/s) from a non-sink, generalized equation.  This data is from (n=5) fluorescein (MW~370 Da) transport experiments in the Tecan (time vs. receiver well concentration (Cr) values).  In this first set of data, I used polycarbonate and polyester transwells, at room temperature, without…

YAP/TAZ YAP/TAZ intracellular localization, and subsequent transcriptional activity, has indicated its role in pathways of mechanotransduction. Investigation of the effects of substrate stiffness on MSC growth and differentiation point to YAP/TAZ as an indicator of how cells are perceiving their mechanical microenvironment and a regulator of the cells’ response to this environment. MSCs cultured on stiff…

This is a recent paper in Nature Materials that shows stem cells are influenced by prior culture on varying stiffness substrates. This means that when cells are induced towards adipogenic or osteogenic lineages, their prior culture history on a stiff or soft substrate affects their future differentiation. This is further evidence that in vitro culture of stem…