HUVEC spreading on pncSi and glass
Following time lapse movies show the spreading of HUVECs on pncSi and glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~4 hrs.
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Looks great.
So we have fibroblasts on glass and HUVEC on pnc-Si. I guess we have a couple more to go?
Make sure you can submit your to the publisher at the same play rate and same duration to make the point that pncSi = glass when it comes to cell spreading.
Yes, I am left with HUVEC on glass and fibroblast on pncSi. I tried fibroblast on pncSi, but it doesn’t look great as the seeding density was high.
I got that point and will make sure the movies are ready for submission.