Burst pressures for new wafers

Kevin was kind enough to share his presentation with us from his visit this July:   Here is the link on Owncloud:   http://drive.bbe.23a.myftpupload.com/public.php?service=files&t=c3eceae62439a478b4d1b676924e45ee

Motivated by Tom’s suggestion at lab meeting on Tuesday, I tried “conditioning” the DMEM+10%FBS before adding the chips.  “Conditioning” just means that I allowed the DMEM+10%FBS to equilibrate in the incubator for an hour before adding the chips to test if a brief (~1 hour) exposure to very high pH causes discoloration.  DMEM+10%FBS without the…

As I am hoping to promote tighter junctions between endothelial cells in my device, I am trying to create an environment that produces shear stress on the endothelial cells. Shear stress up-regulates the pathways that promote tight junctions (Tzima et al 2005, Dejanna et al 2004). The value of the quantity is somewhere around 6…

One of the reviewers for the diffusion based separations paper was concerned that there would be osmotic pressure which would cause convection across the membrane.  Previously we have been aware of osmosis in the DNA experiments by Paul Black and Bill Bernhard, although their results were due to a salt imbalance if I recall correctly….

I oxidized 2 intact chips from w639 using the following protocol: 10min with secondary generator, 15min with both primary and secondary generators.  This protocol is slightly different than my normal protocol (10min 2nd, 5min 1st+2nd, 10min 2nd) because I messed up the settings, although the current protocol is arbitrary anyhow since we haven’t tested it…

Even though we don’t have defect free membranes, I wanted to tweak the gold setup using membranes with pinholes. Previously I was using the small scale diffusion chamber that I use for protein separations. This setup has a retentate of 2-3 uL and a filtrate of 60 uL. To do particle sizing using the Malvern…