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Variation of TEER on the active area and membrane geometry
[Background read – https://trace-bmps.org/blog/theory/2015/01/28/why-teer-values-are-multiplied-by-membrane-area-dc-version] I am putting the excerpt of this old post from Jim in this post. Tom helped me in gathering this data. In order to prove my point that electrical resistance is very sensitive to the membrane geometry, I decided to perform some proof-of-concept experiments with regular transwells. I used 3…
INT results from 4/3/13
I brought some 20nm and 40nm Sepcons to Integrated NanoTechnologies last Wednesday. First, we passed some short radio-labeled DNA strands through a 40 nm Sepcon, and then some longer strands. Both were able to pass through the membrane with a transmission rate of ~70% (as measured by scintillation), and the length of the strand did…
Characterizing Tangential Flow Filtration with Ultra-thin Membranes: A Preliminary Look
Introduction: Over the past few weeks I’ve begun working on a project that deals specifically with characterizing tangential flow filtration with our labs ultra-thin membranes. Essentially, I’m working on understanding the advantages our membranes have over conventional/thicker ones by looking at characteristics such as transmembrane pressure, fouling, and flux. Our working hypothesis is that our…
hiPSC Differentiation to Endothelial Cell Culture Method, Brain Microvascular Endothelial Cells (EECM-BMEC-like cells), Round 1
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and develop strategies that protect against cognitive decline. To fill this gap, we are developing a…
Dye Diffuses through UV-Ozone Treated and Untreated SC267 Samples
ByMike HIn my last post I presented the results of an experiment in which it appeared that BSA did not diffuse through a SC267 sample treated in the UV-Ozone system for 20min at 150C. As a control I also ran BSA dissusion through an untreated SC267 sample and showed that BSA did move across the membrane….
RTP Treated Membrane Stability in DMEM
(Transferred from old blog) Wafer 303 samples, both non-RTP and RTP (800C for 5 minutes), were tested in cell media (DMEM supplemented with 10% FBS) or deionized water, and placed in the incubator (37C 5% CO2) or oven (approximately 37C). Discoloration, pH, and the bead assay were measured initially and every 24 hours. Deionized water…