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More low voltage EO
Continuation of the work with the Borkholder group (see this post). We put together a couple of videos of EO flow in the streaming potential cell today. Runs were performed using Ag/AgCl painted wires at ~2.5 V. Lower voltages weren’t producing very convincing flow rates. Here’s the best video of pumping we obtained: Rec0003_comp The…
Using UV Ozone to Bond PDMS to Glass
ByMike HI talked with Jim last Friday about a second possible dialysis system using PDMS bound to a glass cover slip. I made a batch of PDMS and let it cure at room temperature over the past few days. Jim had suggested I try bonding the PDMS to a glass cover slip using the UV/Ozone system…
Cell Culture with the CO2 nanomembrane Devices
Cell Culture on the CO2 exchange Devices The system becomes more complicated with the addition of cells. Tejas Khire cultured HUVECS in a device that had an inverted design to the one pictured above. (The cells were plated on the top portion (well side) of the nanomembrane with media and the bottom (flat side)of the membrane was…
Microporous Silicon Dioxide (TEOS) vs. Nitride Imaging
After a false start with non-microporous TEOS chips, I quickly did an image comparison with truly porous chips using polystyrene beads. The TEOS and Silicon Nitride chips were both fabricated in the single-well CytoVu format (single 2 mm window, 5.4mm die size). The TEOS chips allows water and media with serum to much more easily…
BSA does not pass through Peg-lyated or EA treated Sepcons with cutoffs of either 10 or 15nm
On Wednesday we ran several separations using both the EA and PEG treated membranes. The treatment process had damaged many of the chips. Of the 10 treated 10nm PEG chips, 2 survived. Of the 10 treated 15nm PEG chips, 2 survived. Of the 10 treated 10nm EA chips 8 survived. Of the 10 treated…
Particle Capture on Pores
ByKileanLast summer, Tejas and I spent a few days beating our heads against the wall because I was trying to develop a mathematical model of particle capture. Specifically, I was trying to understand what amount of transmembrane pressure we would need to hold a particle on (or in, as we are a politically correct, non-discrimination…